Proteins cannot be transferred to a membrane after being stained and de-stained with the Product RPROB, Reversible Protein Detection Kit.
usage
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
technique(s)
protein staining: suitable
storage temp.
2-8°C
Quality Level
1 of 4
이 품목 | GLYCOPRO | PROTSIL2 | G1041 |
|---|---|---|---|
| usage sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels) | usage kit sufficient for 10 mini-gels (10 × 10 cm), kit sufficient for 5 gels or membranes (16.5 × 19 cm) | usage kit sufficient for 25 mini-gels (10 × 10 cm) | usage - |
| technique(s) protein staining: suitable | technique(s) - | technique(s) protein staining: suitable | technique(s) protein staining: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 20-25°C | storage temp. 2-8°C |
Application
R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels[1] with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.
Other Notes
Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
저장 등급
8B - Non-combustible corrosive hazardous materials
Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)
문서
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
프로토콜
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
관련 콘텐츠
Instructions
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When using Product RPROB, Reversible Protein Detection Kit, to stain protein on PAGE gels, can the protein be transferred from the gel to a membrane after destaining?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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