LSKMAGS15
PureProteome Magnetic Stand, 15 mL
The PureProteome Magnetic Stand, 15 mL is designed for use with PureProteome Magnetic Beads in affinity purifications (e. g., His-tag purifications or immunoprecipitations).
Sinónimos:
Magnetic Stand for 15 mL Tubes, Magnetic Stand for PureProteome Magnetic Beads
Seleccione un Tamaño
Acerca de este artículo
Saltar a
material
self-standing
feature
binder
manufacturer/tradename
PureProteome
technique(s)
RNA purification: suitable (with magnetic beads)
protein purification: suitable
shipped in
ambient
Categorías relacionadas
General description
Application
- purification with magnetic beads
- protein purification
- in the purification of the heat shock protein 90 (Hsp90)/ Cdc37 (cell division cycle 37)/ Cyclin-dependent kinase 4 (Cdk4) complex to incubate and wash the beads with 10 bed volumes of lysis buffer[1]
Other Notes
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Contenido relacionado
Read an automated protocol for protein purification using PureProteome™ nickel magnetic beads on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico