Merck
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ENDOLYSS-RO

Roche

Endoproteinase Lys-C Sequencing Grade

from Lysobacter enzymogenes

Synonym(s):
lys-c, protease
Enzyme Commission number:

formulario

lyophilized

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

mol peso

33 kDa by SDS-PAGE (reducing)

envase

pkg of 3 × 5 μg (11047825001)
pkg of 5 μg (11420429001)

manufacturer/tradename

Roche

pH óptimo

8.5-8.8

pH range

5-12

temp. de almacenamiento

2-8°C

Descripción general

Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease.

Especificidad

This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.

Aplicación

Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.[1][2][3][4]

Nota de preparación

Storage conditions (working solution): +2 to +8 °C

Reconstitución

Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 2

Punto de inflamabilidad F

does not flash

Punto de inflamabilidad C

does not flash

Certificate of Analysis

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Certificate of Origin

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