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Bromophenol Blue

ACS reagent

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
Empirical Formula (Hill Notation):
Número de CAS:
Peso molecular:
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ID de la sustancia en PubChem:

Nivel de calidad



ACS reagent




titration: suitable

visual transition interval

3.0-4.6, yellow to blue


273 °C (lit.)


ethanol: 0.1%


diagnostic assay manufacturing

temp. de almacenamiento

room temp

SMILES string




InChI key


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Descripción general

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.


Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.


5, 25 g in glass bottle

Código de clase de almacenamiento

11 - Combustible Solids



Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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