114391

Bromophenol Blue

ACS reagent

• Sinónimos: 3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
• Fórmula empírica (notación de Hill): C₁₉H₁₀Br₄O₅S
• Número CAS: 115-39-9
• Peso molecular: 669.96
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24847169
• EC Number: 204-086-2
• Beilstein/REAXYS Number: 61698
• MDL number: MFCD00005875

Propiedades

• Nombre del producto: Bromophenol Blue, ACS reagent
• InChI key: UDSAIICHUKSCKT-UHFFFAOYSA-N
• InChI: 1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
• SMILES string: Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
• grade: ACS reagent
• form: powder
• technique(s): titration: suitable
• visual transition interval: 3.0-4.6, yellow to blue
• mp: 273 °C (lit.)
• solubility: ethanol: 0.1%
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• Quality Level: 200

Descripción

Application

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)