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Merck

D8284

Bicinchoninic acid disodium salt hydrate

≥98% purity (HPLC), powder

Sinónimos:

2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt, 4,4′-Dicarboxy-2,2′-biquinoline disodium salt, BCA, Disodium 2,2′-biquinoline-4,4′-dicarboxylate

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1 G

MXP 2,262.00

5 G

MXP 6,526.00

10 G

MXP 11,290.00

25 G

MXP 24,692.00

MXP 2,262.00

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Fórmula empírica (notación de Hill):
C20H10N2Na2O4 · xH2O
Número CAS:
979-88-4
Peso molecular:
388.28 (anhydrous basis)
MDL number:
MFCD10567219
UNSPSC Code:
12171500
PubChem Substance ID:
24894202
NACRES:
NA.47

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Nombre del producto

Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)

Quality Level

200

assay

≥98% (HPLC)

form

powder

technique(s)

titration: suitable

color

white to light yellow

solubility

0.5% sodium bicarbonate: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

O.[Na+].[Na+].[O-]C(=O)c1cc(nc2ccccc12)-c3cc(C([O-])=O)c4ccccc4n3

InChI

1S/C20H12N2O4.2Na.H2O/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;;/h1-10H,(H,23,24)(H,25,26);;;1H2/q;2*+1;/p-2

InChI key

FAFVGCAYKZKOPT-UHFFFAOYSA-L

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Este artículo
39177894257SML3593
Bicinchoninic acid disodium salt hydrate ≥98% (HPLC)

Sigma-Aldrich

D8284

Bicinchoninic acid disodium salt hydrate

2,2′-Biquinoline-4,4′-dicarboxylic acid dipotassium salt trihydrate 99%

Sigma-Aldrich

391778

2,2′-Biquinoline-4,4′-dicarboxylic acid dipotassium salt trihydrate

Benzophenone-9 analytical standard

Supelco

94257

Benzophenone-9

Hexachlorophene ≥95% (HPLC)

Sigma-Aldrich

SML3593

Hexachlorophene

technique(s)

titration: suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

color

white to light yellow

color

-

color

-

color

white to beige

solubility

0.5% sodium bicarbonate: 50 mg/mL

solubility

-

solubility

-

solubility

DMSO: 2 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

cleaning products
cosmetics
environmental
food and beverages
personal care

application(s)

-

form

powder

form

powder

form

-

form

powder

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

Application

  • Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.[1]
  • It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).[2]
  • It has been used for determining protein concentration.[3]

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.[4]

related product

Referencia del producto
Descripción
Precios

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Clase de almacenamiento

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000393754
0000393754
0000492542
0000492542
0000468425

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Prevention of rt-PA induced blood-brain barrier component degradation by the poly(ADP-ribose)polymerase inhibitor PJ34 after ischemic stroke in mice.
Teng F, et al.
Experimental Neurology, 248, 416-428 (2013)
Detection and characterization of chitinases and other chitin-modifying enzymes.
Howard MB, et al.
Journal of Industrial Microbiology & Biotechnology, 30, 627-635 (2003)
Ramya Kumareswaran et al.
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 116(3), 388-391 (2015-04-07)
Solid tumors contain regions of chronic and cyclic hypoxia. Chronic hypoxia can downregulate RAD51 and sensitize cells to PARP inhibition. Herein, we show that RAD51 expression, cell survival and toxicity to PARP inhibition is not affected under cyclic hypoxic conditions.
Makon-Sébastien Njock et al.
Blood, 125(20), 3202-3212 (2015-04-04)
The blood contains high concentrations of circulating extracellular vesicles (EVs), and their levels and contents are altered in several disease states, including cardiovascular disease. However, the function of circulating EVs, especially the microRNAs (miRNAs) that they contain, are poorly understood.
P K Smith et al.
Analytical biochemistry, 150(1), 76-85 (1985-10-01)
Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in
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