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Merck

B9410

BIS-TRIS propano

BioXtra, ≥99.0% (titration)

Sinónimos:

1,3-bis[tris(hidroximetil)metilamino]propano

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25 G

MXP 3,690.00

100 G

MXP 9,392.00

250 G

MXP 22,407.00

MXP 3,690.00


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Fórmula lineal:
CH2[CH2NHC(CH2OH)3]2
Número CAS:
Peso molecular:
282.33
Beilstein/REAXYS Number:
1786109
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

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product line

BioXtra

Quality Level

assay

≥99.0% (titration)

form

powder

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

10.0-12.0 (1 M in H2O)

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.05 at 280 in H2O at 1 M
≤0.1 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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1 of 4

Este artículo
B6755137269B7535
assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

-

assay

≥98.0% (titration)

product line

BioXtra

product line

-

product line

EMPROVE® EVOLVE

product line

BioXtra

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing
general analytical
life science and biopharma

application(s)

-

application(s)

diagnostic assay manufacturing

form

powder

form

powder

form

powder

form

powder

solubility

H2O: 1 M, clear, colorless

solubility

water: 30 % (w/w), clear, colorless

solubility

-

solubility

H2O: 1 M, clear, colorless

Application

  • A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood.: BIS-TRIS propane is used as a buffering agent in the capillary electrophoresis method to assess global DNA methylation levels in human whole blood. The buffer′s role is crucial in maintaining the stability and reproducibility of the electrophoretic conditions (Zinellu et al., 2019).

  • Low potential manganese ions as efficient electron donors in native anoxygenic bacteria.: BIS-TRIS propane is used to buffer the experimental conditions in studying the role of low potential manganese ions as electron donors in bacteria. The findings enhance our understanding of electron transfer processes in microbial systems (Deshmukh et al., 2018).

  • Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection.: This research uses BIS-TRIS propane in a capillary electrophoresis method to evaluate DNA methylation levels. The buffer′s properties ensure accurate and reliable detection, which is crucial for epigenetic studies (Zinellu et al., 2017).

Other Notes

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Clase de almacenamiento

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Hamed Tahmasbi et al.
Journal of pharmaceutical and biomedical analysis, 171, 1-7 (2019-04-07)
Secreted recombinant activated clotting factor VII activated (rFVIIa) in cell culture media missing gamma-carboxyglutamic acid (Gla) domain as a result of failure in gamma-carboxylation or cell lysis is called Gla-domainless impurity which has less negative charge compared to native rFVIIa.
Sami J O Varjo et al.
Journal of chromatography. A, 1081(1), 92-98 (2005-07-15)
Greater stability of liposome coatings and improved resolution of model steroids in capillary electrochromatography (CEC) were sought by adding small diamines (ethylenediamine, diaminopropane, bis-tris-propane, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid, HEPES)) to the liposome solution before coating of fused silica capillaries. The phospholipid
S J Smith et al.
The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)
The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)
R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
Michael C Breadmore
Journal of chromatography. A, 1217(24), 3900-3906 (2010-05-11)
An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could

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