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Merck

E1510

Ethidium bromide solution

BioReagent, Molecular Biology, 10 mg/mL in H2O

Sinónimos:

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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10 ML

MXP 1,958.00

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Fórmula empírica (notación de Hill):
C21H20BrN3
Número CAS:
1239-45-8
Peso molecular:
394.31
Beilstein/REAXYS Number:
3642536
MDL number:
MFCD00011724
UNSPSC Code:
41105322
PubChem Substance ID:
24894418
NACRES:
NA.31

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grade

Molecular Biology

product line

BioReagent

concentration

10 mg/mL in H2O

technique(s)

electrophoresis: suitable

suitability

suitable for gel electrophoresis

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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Este artículo
E1385E763746067
Ethidium bromide solution BioReagent, Molecular Biology, 10 mg/mL in H2O

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Ethidium bromide solution BioReagent, Molecular Biology, 500 μg/mL in H2O

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Ethidium bromide BioReagent, Molecular Biology, powder

Sigma-Aldrich

E7637

Ethidium bromide

Ethidium bromide solution suitable for fluorescence, ~1% in H2O

Sigma-Aldrich

46067

Ethidium bromide solution

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

-

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for fluorescence

concentration

10 mg/mL in H2O

concentration

500 μg/mL in H2O

concentration

-

concentration

~1% in H2O

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Ethidium bromide solution has been used:
  • as a stain to visualize U937 cells to assess cell viability[1]
  • to detect polymerase chain reaction products[2]
  • in agarose gel electrophoresis based gel retardation assay[3]

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Preparation Note

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Other Notes

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pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

hcodes

H331,H341

Hazard Classifications

Acute Tox. 3 Inhalation - Muta. 2

Clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SHBR6796
SHBP1050
SHBM6092
MKCL8014
MKCJ2002

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Quinones GA, et al.
Blood, 121(7), e25-e33 (2013)
Keith E Herold et al.
Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)
A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling
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The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculative. Here we
Ulrich Braunschweig et al.
Genome research, 24(11), 1774-1786 (2014-09-27)
Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron retention (IR) is the least well understood. In plants and unicellular eukaryotes, IR is
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      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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