MABF2771

Anti-564/idiotype Antibody, clone 9D11

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 9D11, monoclonal
• mol wt: observed mol wt ~54 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100 μL
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: Unknown
• UniProt accession no.: N/A
• shipped in: ambient
• target post-translational modification: unmodified

Description

General description

An idiotype is the genetically determined variation of structures in the variable regions of immunoglobulins. Anti-idiotypic antibodies are known to target specific epitopes, on another antibody that are unique for that target antibody. The collection of epitopes for a given antibody defines its idiotype and is generally found in the complementarity determining regions of that targeted antibody. These antibodies can also bind shared epitopes on highly related monoclonal antibodies. Their specificity allows for the specific detection of antibody of interest even in the presence of endogenous polyclonal antibody. Due to their specificity for the variable region, these antibodies can also be useful in pharmacokinetic and immunogenicity studies. 564Igi is shown to be a reliable murine model of systemic lupus erythematous (SLE). These mice develop SLE profile closely resembling that of human SLE. These mice possess rearranged immunoglobulin heavy- and light-chain genes inserted into their respective loci that are together specific for nucleolar antigens. The 564 antibody is reported to have a characteristic idiotype and B cells carrying the corresponding B-cell antigen receptor are idiotype positive. The epitope recognized by the 564 idiotype antibody is a domain that may be common to multiple self-antigens. (Ref.: Berland, R., et al. (2006). Immunity 25(3); 429-440).

Immunogen

An idiotype of a mouse IgG (clone C11) representing B cell receptor expressed by auto reactive B cells in 564 Igi mice.

Application

Quality Control Testing

Evaluated by Western Blotting in Idiotype of a mouse IgG target antigen (clone C11).

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected idiotype of a mouse IgG target antigen (clone C11) .

Tested Applications

Flow Cytometry Analysis: A representative lot detected 564/idiotype in Flow Cytometry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450; van der Poel, C.E., et al. (2019). Cell Rep. 29(9):2745-2755.e4; Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

Immunohistochemistry Applications: A representative lot detected 564/idiotype in Immunohistochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunoprecipitation Analysis: A representative lot detected 564/idiotype in Immunoprecipitation applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunocytochemistry Analysis: A representative lot detected 564/idiotype in Immunocytochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunofluorescence Analysis: A representative lot detected 564/idiotype in Immunofluorescence applications (Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-564/idiotype, clone 9D11, Cat. No. MABF2771, is a mouse monoclonal antibody that detects mouse IgG idiotype and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, , Immunoprecipitation, & Western Blotting.

Biochem/physiol Actions

Clone 9D11 is a mouse monoclonal antibody that detects idiotype of a mouse IgG.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1