206865

Primuline

Dye content 50 %

• Synonym(s): Direct Yellow 59, Primuline Yellow
• Empirical Formula (Hill Notation): C₂₁H₁₄N₃NaO₃S₃
• CAS Number: 8064-60-6
• Molecular Weight: 475.54
• Colour Index Number: 49000
• MDL number: MFCD00005798
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852417
• NACRES: NA.47

Properties

• form: powder or crystals
• Quality Level: 100
• composition: Dye content, 50%
• mp: >300 °C
• λmax: 229 nm (2nd); 340-355 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].Cc1ccc2nc(sc2c1S([O-])(=O)=O)-c3ccc4nc(sc4c3)-c5ccc(N)cc5
• InChI: 1S/C21H15N3O3S3.Na/c1-11-2-8-16-18(19(11)30(25,26)27)29-21(24-16)13-5-9-15-17(10-13)28-20(23-15)12-3-6-14(22)7-4-12;/h2-10H,22H2,1H3,(H,25,26,27);/q;+1/p-1
• InChI key: RSRNHSYYBLEMOI-UHFFFAOYSA-M

Description

Application

Primuline has been used as a reagent in the preparation of lipids for chloroplast lipid membrane.[1] It has been used as a dye in preparative thin-layer chromatography (TLC).[2]

Biochem/physiol Actions

Primuline, also known as direct yellow 59 or primuline yellow, is considered to be a more sensitive and non-destructive dye for lipids. It is widely used as a fluorescent dye in preparative thin-layer chromatography (TLC). Primuline is suitable for vital staining. It is used as a fluorochrome in plant tissues. Primuline is also used to stain pollen grains and lignified cell walls. In addition, it also helps in segregating intact and broken starch grains. At pH 8, primuline acts as a fluorochrome for mast cells.[3][4]

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves