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Merck

A0802

Adipic acid dihydrazide–Agarose

saline suspension

Synonym(s):

Adipic acid dihydrazide agarose beads

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About This Item

MDL number:
MFCD00161954
UNSPSC Code:
23151817
NACRES:
NA.56

Key Documents

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biological source

plant

form

saline suspension

extent of labeling

≥8 μmol per mL

technique(s)

microbe id | metabolite detection: suitable

matrix activation

cyanogen bromide

matrix spacer

11 atoms (when ligands (aldehydes, carboxylic acids) are coupled through free hydrazide groups)

suitability

suitable for chromatography

storage temp.

2-8°C

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This Item
8.416898.18650S1638
Adipic acid dihydrazide–Agarose saline suspension

Sigma-Aldrich

A0802

Adipic acid dihydrazide–Agarose

Adipic dihydrazide for synthesis

Sigma-Aldrich

8.41689

Adipic dihydrazide

Adipic acid for synthesis

Sigma-Aldrich

8.18650

Adipic acid

Streptavidin−Agarose from Streptomyces avidinii buffered aqueous suspension

Millipore

S1638

Streptavidin−Agarose from Streptomyces avidinii

biological source

plant

biological source

-

biological source

-

biological source

-

technique(s)

microbe id | metabolite detection: suitable

technique(s)

-

technique(s)

-

technique(s)

affinity chromatography: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

saline suspension

form

powder

form

crystals

form

buffered aqueous suspension

suitability

suitable for chromatography

suitability

-

suitability

-

suitability

-

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

2-30°C

storage temp.

2-8°C

Application

Adipic acid dihydrazide Agarose can be used in proteomics and protein chromatography. It has been utilized in research for purifying and identifying the fungal phytotoxin Fusicoccin (FC), identifying RNA binding proteins that interact with RNA cis-elements, and enzyme purification such as peroxisomes from guinea pig liver.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000502313
0000502313
0000486121
0000486121
0000455097

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M Caputi et al.
The EMBO journal, 18(14), 4060-4067 (1999-07-16)
Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are
S C Datta et al.
The Journal of biological chemistry, 265(14), 8268-8274 (1990-05-15)
The peroxisomal acyl/alkyl dihydroxyacetone-phosphate reductase (EC 1.1.1.101) was solubilized and purified 5500-fold from guinea pig liver. The enzyme could be solubilized by detergents only at high ionic strengths in presence of the cosubstrate NADPH. Peroxisomes, isolated from liver by a
Alice Tianbu Zhang et al.
Journal of cell science, 124(Pt 12), 2058-2069 (2011-05-26)
Non-coding Y RNAs are required for the initiation of chromosomal DNA replication in mammalian cells. It is unknown how they perform this function or if they associate with a nuclear structure during DNA replication. Here, we investigate the association of
D Grate et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(11), 6131-6136 (1999-05-26)
The biological function of specific gene products often is determined experimentally by blocking their expression in an organism and observing the resulting phenotype. Chromophore-assisted laser inactivation using malachite green (MG)-tagged antibodies makes it possible to inactivate target proteins in a
Ruben Hovhannisyan et al.
BioTechniques, 46(2), 95-98 (2009-03-26)
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation. These purifications can be complicated by residual RNase activity in cellular extracts that can degrade
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