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A6103
Aprotinin
recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay
Synonym(s):
BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-994-9
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Product Name
Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)
recombinant
expressed in Nicotiana (tobacco)
description
recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)
product line
BioUltra
assay
≥98% (SDS-PAGE)
form
powder
specific activity
≥5 TIU/mg protein
mol wt
~_6.5 kDa
technique(s)
inhibition assay: suitable
solubility
0.85% sodium chloride: 5 mg/mL
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
cow ... PTI(404172)
Related Categories
1 of 4
This Item | A6106 | A1153 | A4529 |
|---|---|---|---|
| assay ≥98% (SDS-PAGE) | assay ≥98% (SDS-PAGE) | assay - | assay - |
| Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility 0.85% sodium chloride: 5 mg/mL | solubility H2O: 5 mg/mL, clear to very slightly hazy | solubility H2O: ≥5 mg/mL | solubility H2O: >10 mg/mL |
Other Notes
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.
Preparation Note
Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Protocols
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Related Content
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What is the Department of Transportation shipping information for this product?
1 answer-
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How does Product A6103, Aprotinin bovine, compare to Trasylol?
1 answer-
Trasylol is the Bayer AG registered trademark name for this material.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How should I store solutions of Product A6103, Aprotinin bovine?
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We generally advise storing stock solutions of approximately 5-10 mg/mL. Working solutions down to 0.065 micrograms/mL can be stored refrigerated at 2-8°C for one week, or at - 20°C for appoximately six months in single use aliquots. See Table 2 in the Datasheet for details.
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Do you assay this the activity of Product A6103, Aprotinin bovine, in TIU or in KIU?
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We express the product's activity in TIU (Trypsin Inhibitor Units). Multiply the activity on the Certificate of Analysis by 1300 to convert it to KIU (Kallikrein Inhibitor Units).
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Product A6103, recombinant bovine aprotinin has a specification of ~60% protein. What is the remaining components of the solid?
1 answer-
Product A6103, recombinant bovine aprotinin is >98% purity by SDS-PAGE. The product is ~60% protein with the balance being salts (NaCl).
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At what concentration should I use Product A6103, Aprotinin bovine?
1 answer-
For most applications, 10 to 800 nM works well (based on a molecular weight of 6512 g/mol). This information may be found in the technical bulletin for the Protease Inhibitor Panel, Product No. INHIB1.
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