The protein concentration of Product No. P5381 (PGI) can vary from lot-to-lot. It is typically in the range of 5-20 mg protein per mL. The lot-specific concentration will be given on the certificate of analysis.
P5381
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)
Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)
Synonym(s):
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-603-1
MDL number:
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Product Name
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae), Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)
biological source
bakers yeast
type
Type III
form
ammonium sulfate suspension
specific activity
≥400 units/mg protein (biuret)
mol wt
tetramer 119,500 Da±600
concentration
≥3 mg/mL protein Biuret
technique(s)
activity assay: suitable
color
white
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
yeast ... PGI1(852495)
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This Item | F2668 | T2507 | U8501 |
|---|---|---|---|
| specific activity ≥400 units/mg protein (biuret) | specific activity - | specific activity ~10,000 units/mg protein | specific activity ≥40 units/mg protein |
| Gene Information yeast ... PGI1(852495) | Gene Information - | Gene Information - | Gene Information - |
| biological source bakers yeast | biological source - | biological source - | biological source bakers yeast |
| technique(s) activity assay: suitable | technique(s) photometry: suitable | technique(s) - | technique(s) - |
| concentration ≥3 mg/mL protein Biuret | concentration - | concentration - | concentration - |
| form ammonium sulfate suspension | form - | form ammonium sulfate suspension | form lyophilized powder |
Application
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
- to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.[1]
- as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.[2]
- to convert fructose to glucose while evaluating fructose content in kudzu roots.[3]
Biochem/physiol Actions
Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP).[4] It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.[5]Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.[4]
General description
Other Notes
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.
Physical form
Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Phosphoglucose Isomerase Is Important for Aspergillus fumigatus Cell Wall Biogenesis
Zhou Y, et al.
mBio, 13(4) (2022)
Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
Yeast Models of Phosphomannomutase 2 Deficiency, a Congenital Disorder of Glycosylation
Lao JP, et al.
G3 (Bethesda, Md.), 9(2), 413-423 (2019)
Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14
Yanagawa T, et al.
Cancer Research, 67(18), 8682-8689 (2007)
S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Related Content
QC Methods
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What is the typical protein concentration for Product No. P5381 (PGI)?
1 answer-
Helpful?
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What is the KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate, and what does that mean?
1 answer-
KM is the concentration of substrate that leads to half-maximal velocity. The KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate is 0.167, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the pH optimum of Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?
1 answer-
The pH optimum for yeast PGI (Product No. P5381) is 7, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).
Helpful?
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What is the substrate for Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?
1 answer-
The substrate for PGI is D-fructose-6-phosphate, according to our Enzyme Assay procedure.
Helpful?
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