17-344

Zestaw do oznaczania fosforylacji H2A.X (cytometria przepływowa)

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X.

• Synonim(y): Test dla H2A.X, Zestaw do fosforylacji H2A.X, Zestaw do testów cytometrii przepływowej
• UNSPSC Code: 12161503
• NACRES: NA.32
• eCl@ss: 32161000

Właściwości

• Quality Segment: 100
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable, flow cytometry: suitable
• NCBI accession no.: NM_002105.2
• UniProt accession no.: P16104
• detection method: fluorometric
• shipped in: dry ice

Opis

General description

Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X.

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.

Application

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Packaging

100 assays

Analysis Note

phosphorylation of Histone H2A.X

Other Notes

Anti-phospho H2A.X, FITC conjugate

Normal Mouse IgG, FITC conjugate (Cat.# 12-487)

16X Fixation Solution

10X Permeabilization Solution

10X Wash Solution

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Supply conditions do not apply to the regions and states of Brazil: North, Northeast, Mato Grosso do Sul, Mato Grosso, and Rio Grande do Sul.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS06,GHS08,GHS05
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H225,H301 + H311,H314,H317,H330,H335,H341,H350,H370
• Ostrzeżenia: P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• Narządy docelowe: Eyes,Central nervous system, Respiratory system
• Klasa składowania: 3 - Flammable liquids