This product is prepared at a concentration of 1 U/ul. A milligram concentration is not available. This RNase I solution also contains 10mM Hepes, pH 7.6, 10mM MgCl2, 10mM CaCl2, and 50% glycerol
69182
DNase I, RNase free
For applications in which maintenance of RNA integrity is critical
Synonim(y):
RNase-free DNase I
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1000 U | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 587,00 zł |
Informacje o tej pozycji
NACRES:
NA.85
UNSPSC Code:
12352202
587,00 zł
Dostępne do wysyłki DZISIAJSzczegóły
Pomoc techniczna
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Pozwól nam pomócform
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
technique(s)
DNA extraction: suitable
input
general use sample(s)
suitability
suitable for nucleic acid purification
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.
Other Notes
One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Ta pozycja | |||
|---|---|---|---|
| description For applications in which maintenance of RNA integrity is critical | description - | description Novagen® | description Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. |
| technique(s) DNA extraction: suitable | technique(s) - | technique(s) - | technique(s) immunoblotting: suitable |
| form liquid | form liquid | form liquid | form liquid |
| suitability suitable for nucleic acid purification | suitability - | suitability - | suitability - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) sample preparation |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| storage condition avoid repeated freeze/thaw cycles, OK to freeze | storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze |
Klasa składowania
10 - Combustible liquids
Co się dzieje?
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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What is the concentration of this product in µg/mL?
1 answer-
Helpful?
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Does DNase 1 (SKU 69182 and others) effectively work on circular DNA?
1 answer-
I came across a research article on ResearchGate that discusses DNase I and its effects on plasmid DNA. The article provides the following insights:
1) DNase I can degrade plasmid DNA, and its activity depends on the ionic strength of the reaction buffer. The enzyme exhibits optimal activity in a buffer containing Mg2+ and Ca2+. Micromolar levels of Ca2+ act as an enzyme activator in the presence of Mg2+. It is also mentioned that sample buffer interference (TE buffer), which chelates Ca2 and Mg2, might have affected the DNase treatment on pure plasmid.
2) The suggestion is to use a buffer containing Mg2+ and Ca2+, increase the concentration of DNase I, and incubate at 37°C for 10 minutes.
3) For circular DNA, the article recommends denaturing the circular DNA first, as DNase I degrades DNA by making random single-strand nicks in the phosphate backbone. When the DNA is circular, the fragments are likely to remain coiled together. To linearize the plasmid, two nicks should overlap, which is a rare event. The article suggests using denaturing agents such as urea to facilitate the unfolding of the plasmid or linearizing the plasmid with a specific primer before the DNase I treatment. It also questions the need to digest the plasmid to remove RNA, suggesting the use of an RNA-specific RNAse such as RNAse A.
Helpful?
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