AB1530
Anti-Peripherin Antibody
serum, Chemicon®
Synonim(y):
Anty-NEF4, Anty-PRPH1
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Wybierz wielkość
100 μL
2250,00 zł
2250,00 zł
Sprawdź dostępność w koszyku
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1174
Informacje o tej pozycji
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Przejdź do
Pomoc techniczna
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Pozwól nam pomócpochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat, human, pig, bovine, mouse
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... PRPH2(5961)
1 of 4
Ta pozycja | P5117 | AB9282 | SAB1401314 |
|---|---|---|---|
| biological source rabbit | biological source mouse | biological source chicken | biological source mouse |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| antibody form serum | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| species reactivity rat, human, pig, bovine, mouse | species reactivity rat, bovine, pig, mouse, rabbit, feline, human | species reactivity rat | species reactivity human |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable | technique(s) immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1.0 μg/mL using total cell extract from rat PC12 cells | technique(s) immunocytochemistry: suitable, western blot: suitable | technique(s) capture ELISA: suitable, immunofluorescence: suitable, western blot: 1-5 μg/mL |
Opis ogólny
56-58 kDa
Peripherin is a 56-58 kDa type III intermediate filament protein (Portier et al. 1983), expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g. Hirschsprung′s disease) states. Abnormal inclusions containing peripherin have also been reported in ALS. During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.
Immunogen
Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990) 57:235-248], containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE.
Zastosowanie
Additional Research Applications
Immunohistochemistry:
A previous lot of this antibody was used at 1:100-1:200 dilution. It is suggested that the antibody be used on frozen sections fixed in acetone at -20°C. AB1530 will work on tissue fixed for one hour or less in fresh 4% paraformaldehyde. It has been reported that this antibody can be used on paraffin embedded tissue sections. See Cell & Tissue Research (1997) 288:11-23 & European J. Dermatology (1998) 8:339-342.
Electron Microscopy:
A previous lot of this antibody was used on Electron Microscopy.
Optimal working dilutions and protocols must be determined by end user.
Immunohistochemistry:
A previous lot of this antibody was used at 1:100-1:200 dilution. It is suggested that the antibody be used on frozen sections fixed in acetone at -20°C. AB1530 will work on tissue fixed for one hour or less in fresh 4% paraformaldehyde. It has been reported that this antibody can be used on paraffin embedded tissue sections. See Cell & Tissue Research (1997) 288:11-23 & European J. Dermatology (1998) 8:339-342.
Electron Microscopy:
A previous lot of this antibody was used on Electron Microscopy.
Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Neuronal & Glial Markers
Sensory & PNS
Neuronal & Glial Markers
This Anti-Peripherin Antibody is validated for use in IH, IH(P), WB for the detection of Peripherin.
Działania biochem./fizjol.
Recognizes Peripherin. The antibody stains a ~57 kDa band cleanly and specifically and does not stain vimentin, GFAP, alpha-internexin or any of the neurofilament subunits. Strong staining on rat, mouse, human, pig and cow peripherin. Does not stain chicken, quail or other more distantly related species which appear to lack peripherin.
Strong staining on rat and human. Cross-reacts with mouse, pig, and bovine. Other species not tested.
Postać fizyczna
Rabbit polyclonal antisera, no preservatives.
Unpurified
Uwaga dotycząca przygotowania
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected peripherin on 10 μg of PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected peripherin on 10 μg of PC12 lysates.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
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Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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