Medulloblastoma is one of the most common malignant brain tumors in children, with the highest number of diagnoses occurring in patients between 6 and 8 years of age (1). Medulloblastomas are subdivided into four subtypes based on molecular genetic characteristics: WNT, sonic hedgehog (SHH), group 3 and group 4 (1). Of these, group 3 is characterized by amplification of the oncogene MYC, one of the most common gene alterations associated with medulloblastoma.
The D425 Med human medulloblastoma cell line is one of the most highly cited cellular models for medulloblastoma and is classified as a subgroup 3, harboring MYC amplification as well as mutant P53 (2). The D425 Med medulloblastoma cell line was derived from tumor cells of 6-year old patient and grows as suspended or lightly-adherent cell clumps in culture. D425 Med medulloblastoma cells are positive for neurofilament proteins but negative for GFAP (3).
<bold>Source:</bold> The D425 medulloblastoma cell line was derived from biopsy of a tumor from a 6-year-old male patient (2).
Research Category: Cancer
Biochem/physiol Actions
Human, Cancer Cells
Packaging
≥1X106 cells/vial
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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