Merck

PMSF-RO

Roche

PMSF

Phenylmethylsulfonyl fluoride

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Synonim(y):
Phenylmethanesulfonyl fluoride, phenylmethylsulfonyl fluoride, α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride
Empirical Formula (Hill Notation):
C7H7FO2S
Numer CAS:
Masa cząsteczkowa:
174.19
Beilstein:
2088311
Numer MDL:
Identyfikator substancji w PubChem:

Poziom jakości

Próba

98% (TLC)

forma

solid

masa cząsteczkowa

174.2

opakowanie

pkg of 10 g (10837091001)
pkg of 25 g (11359061001)

manufacturer/tradename

Roche

stężenie

17-170 μg/mL

technique(s)

western blot: suitable

pKa 

7.0

mp

92-94 °C

rozpuszczalność

>10 mg/mL (Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.)

Warunki transportu

ambient

temp. przechowywania

20-25°C

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

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Ta pozycja
P76269348252332
PMSF Phenylmethylsulfonyl fluoride

Roche

PMSF-RO

PMSF

form

solid

form

powder

form

liquid

form

crystalline solid

mol wt

174.2

mol wt

-

mol wt

-

mol wt

-

mp

92-94 °C

mp

91-94 °C

mp

-

mp

-

solubility

>10 mg/mL (Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.)

solubility

dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.), H2O: unstable

solubility

-

solubility

ethanol: soluble, isopropanol: soluble, methanol: soluble

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

Specyficzność

Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.

Zastosowanie

PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.

Działania biochem./fizjol.

PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.

Jakość

Purity: Chromatographically homogeneous

Uwaga dotycząca przygotowania

Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.

Rekonstytucja

Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.

Inne uwagi

Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
For life science research only. Not for use in diagnostic procedures.

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Przykład

T1503
Numer produktu
-
25G
Wielkość opakowania/ilość

Przykłady dodatkowe:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

wpisz jako 1.000309185)

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