This product has not been specifically validated for use with Revvity Lumina IVIS systems. However, ATTO 647N is expected to be a suitable alternative to Alexa Fluor 647, as its excitation and emission fall within the far-red spectral region, similar to Alexa 647. The spectral properties of ATTO 647N are:
Excitation (λabs): ~644–646 nm
Emission (λem ): ~664–669 nm
Efficient excitation range: ~625–660 nm
Given this spectral overlap, ATTO 647N is generally compatible with Alexa 647 filter settings; however, optimization may be required depending on the instrument configuration and application. For additional technical details, please refer to the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/276/04507dat.pdf