10065

α-Amylase from Aspergillus oryzae

powder, ~30 U/mg

• Synonim(y): 1,4-α-D-Glucan-glucanohydrolase
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-588-1
• NACRES: NA.54
• MDL number: MFCD00081319
• Numer WE: 3.2.1.1 (BRENDA, IUBMB)
• Specific activity: ~30 U/mg

Właściwości

• form: powder
• Quality Segment: 100
• specific activity: ~30 U/mg
• mol wt: M_r ~51000
• storage temp.: 2-8°C

Opis

General description

Microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. α-Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration).

Application

α-Amylase has been used:

• as a control enzyme in agar plate-based and carboxymethylcellulose-based clearing assays to screen cellulase activity
• for the hydrolysis of starch to explore the the role of wheat starch in frozen dough
• to inhibit Staphylococcus aureus biofilms

Biochem/physiol Actions

Aspergillus oryzae α-amylase (Ao α-amylase) enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).

For the determination of fats in food; Application in (selective) hydrolysis/condensation of glycosidic bonds.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302,H334
• pcodes: P261 - P264 - P270 - P284 - P301 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Oral - Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves