Merck
  • 46067
All Photos(1)

46067

Sigma-Aldrich

Ethidium bromide solution

for fluorescence, ~1% in H2O

All Photos(1)
Synonym(s):
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
Empirical Formula (Hill Notation):
C21H20BrN3
CAS Number:
1239-45-8
Molecular Weight:
394.31
Beilstein:
3642536
Numer MDL:
MFCD00011724
Identyfikator substancji w PubChem:
57650684
NACRES:
NA.32

klasa czystości

for fluorescence

Poziom jakości

100

stężenie

~1% in H2O

fluorescencja

λex 480 nm; λem 620 nm in H2O
λex 518 nm; λem 605 nm (bound to DNA)
λex 530 nm; λem 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA)

temp. przechowywania

2-8°C

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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Ethidium bromide solution for fluorescence, ~1% in H2O

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Ethidium bromide solution

Ethidium bromide solution BioReagent, for molecular biology, 500 μg/mL in H2O

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E1385

Ethidium bromide solution

grade

for fluorescence

grade

-

grade

for molecular biology

grade

for molecular biology

concentration

~1% in H2O

concentration

-

concentration

10 mg/mL in H2O

concentration

500 μg/mL in H2O

fluorescence

λex 480 nm; λem 620 nm in H2O, λex 518 nm; λem 605 nm (bound to DNA), λex 530 nm; λem 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA)

fluorescence

-

fluorescence

-

fluorescence

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

Zastosowanie

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Działania biochem./fizjol.

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Rekonstytucja

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

piktogramy

Skull and crossbonesHealth hazard
GHS06,GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H331 - H341

Zwroty wskazujące środki ostrożności

P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313

Klasyfikacja zagrożeń

Acute Tox. 3 Inhalation - Muta. 2

Kod klasy składowania

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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