46067

Ethidium bromide solution

for fluorescence, ~1% in H₂O

• Synonim(y): 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
• Wzór empiryczny (zapis Hilla): C₂₁H₂₀BrN₃
• Numer CAS: 1239-45-8
• Masa cząsteczkowa: 394.31
• Beilstein: 3642536
• Numer MDL: MFCD00011724
• Kod UNSPSC: 12161505
• Identyfikator substancji w PubChem: 57650684
• NACRES: NA.32

Właściwości

• klasa czystości: for fluorescence
• Poziom jakości: 100
• stężenie: ~1% in H₂O
• fluorescencja: λ_ex 480 nm; λ_em 620 nm in H₂O; λ_ex 518 nm; λ_em 605 nm (bound to DNA); λ_ex 530 nm; λ_em 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA)
• temp. przechowywania: 2-8°C
• ciąg SMILES: [Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
• Klucz InChI: ZMMJGEGLRURXTF-UHFFFAOYSA-N

Opis

Zastosowanie

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Działania biochem./fizjol.

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Rekonstytucja

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06,GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H331,H341
• Zwroty wskazujące środki ostrożności: P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313
• Klasyfikacja zagrożeń: Acute Tox. 3 Inhalation - Muta. 2
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter