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Merck

46067

Ethidium bromide solution

suitable for fluorescence, ~1% in H2O

Synonim(y):

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C21H20BrN3
Numer CAS:
1239-45-8
Masa cząsteczkowa:
394.31
UNSPSC Code:
12161505
NACRES:
NA.32
PubChem Substance ID:
57650684
MDL number:
MFCD00011724
Beilstein/REAXYS Number:
3642536

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InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

concentration

~1% in H2O

fluorescence

λex 480 nm; λem 620 nm in H2O, λex 518 nm; λem 605 nm (bound to DNA), λex 530 nm; λem 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

100

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Ta pozycja
E1385E1510E7637
Ethidium bromide solution suitable for fluorescence, ~1% in H2O

Sigma-Aldrich

46067

Ethidium bromide solution

Ethidium bromide solution BioReagent, Molecular Biology, 500 μg/mL in H2O

Sigma-Aldrich

E1385

Ethidium bromide solution

Ethidium bromide solution BioReagent, Molecular Biology, 10 mg/mL in H2O

Sigma-Aldrich

E1510

Ethidium bromide solution

Ethidium bromide BioReagent, Molecular Biology, powder

Sigma-Aldrich

E7637

Ethidium bromide

grade

for fluorescence

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

fluorescence

λex 480 nm; λem 620 nm in H2O, λex 530 nm; λem 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA), λex 518 nm; λem 605 nm (bound to DNA)

fluorescence

-

fluorescence

-

fluorescence

λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;), λex 526 nm; λem 605 nm in aqueous buffer

concentration

~1% in H2O

concentration

500 μg/mL in H2O

concentration

10 mg/mL in H2O

concentration

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Preparation Note

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.
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related product

Numer produktu
Opis
Cennik

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

hcodes

H331,H341

Hazard Classifications

Acute Tox. 3 Inhalation - Muta. 2

Klasa składowania

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certyfikaty analizy (CoA)

Lot/Batch Number
BCCJ8584
BCCG3060
BCCC4195
BCBW2534
BCBQ5502V

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