Merck

E1510

Sigma-Aldrich

Ethidium bromide solution

BioReagent, for molecular biology, 10 mg/mL in H2O

Synonim(y):
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
Empirical Formula (Hill Notation):
C21H20BrN3
Numer CAS:
Masa cząsteczkowa:
394.31
Beilstein:
3642536
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.31

klasa czystości

for molecular biology

Poziom jakości

linia produktu

BioReagent

stężenie

10 mg/mL in H2O

technique(s)

electrophoresis: suitable

przydatność

suitable for gel electrophoresis

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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Ta pozycja
E7637E875146067
Ethidium bromide BioReagent, for molecular biology, powder

Sigma-Aldrich

E7637

Ethidium bromide

Ethidium bromide ~95% (HPLC)

Sigma-Aldrich

E8751

Ethidium bromide

product line

BioReagent

product line

BioReagent

product line

-

product line

-

concentration

10 mg/mL in H2O

concentration

-

concentration

-

concentration

~1% in H2O

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

-

suitability

-

Zastosowanie

Ethidium bromide solution has been used:
  • as a stain to visualize U937 cells to assess cell viability
  • to detect polymerase chain reaction products
  • in agarose gel electrophoresis based gel retardation assay

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Działania biochem./fizjol.

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Rekonstytucja

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

produkt powiązany

piktogramy

Skull and crossbonesHealth hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Acute Tox. 3 Inhalation - Muta. 2

Kod klasy składowania

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable


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Przykład

T1503
Numer produktu
-
25G
Wielkość opakowania/ilość

Przykłady dodatkowe:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

wpisz jako1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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Powiązane treści

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