51309

TRIS borate-EDTA buffer concentrate, long run

for Northern and Southern blotting, solution

• UNSPSC Code: 12161700
• NACRES: NA.25
• MDL number: MFCD00217350

Właściwości

• grade: Molecular Biology
• Quality Segment: 100
• product line: BioReagent
• form: solution
• quality: 10× concentrate, ready-to-use solution
• impurities: phosphatases, none detected, proteases, none detected, DNases, none detected, RNases, none detected
• storage temp.: room temp

Opis

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Preparation Note

The TBE 10x concentrate contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H360FD
• Ostrzeżenia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Klasa składowania: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable