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Trypsin inhibitor from Glycine max (soybean)
lyophilized powder, ~10000 U/mg
Synonim(y):
SBTI
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
250 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 288,00 zł |
1 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 876,00 zł |
Informacje o tej pozycji
288,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
Glycine max (soybean)
Quality Segment
form
lyophilized powder
specific activity
~10000 U/mg
mol wt
Mr ~20000
input
plant, fungi and insect sample(s)
solubility
0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Other Notes
For the inhibition of proteolytic activity; Use in affinity chromatography for the purification of trypsin; Prepared by the method of M. Kunitz.
1 U corresponds to the amount of inhibitor which reduces the trypsin activity by 1 BAEE-U. (1 BAEE-U is the amount of enzyme which increases the absorbance at 253 nm by 0.001 per minute at pH 7.6 and 25 °C; BAEE, Cat. No. 12880, as substrate).
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
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Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder, ~10000 U/mg | description lyophilized powder | description lyophilized powder | description BioReagent, For use as a marker in SDS-PAGE |
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in ambient |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless | solubility H2O: >10 mg/mL, soluble, phosphate buffer: >10 mg/mL (hazy above 10 mg/ml) | solubility concentrate: >10 mg/mL, hazy, amber-yellow, water: 10 mg/mL, phosphate buffer: 10 mg/mL, balanced salt solution: 1 mg/mL, serum-free medium: soluble | solubility phosphate buffer: 10 mg/mL, serum-free medium: soluble, water: 10 mg/mL, balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Klasa składowania
11 - Combustible Solids
WGK
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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What is the Department of Transportation shipping information for this product?
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
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Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
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