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A0855

Anti-Auxin antibody, Mouse monoclonal

clone 1E11-C11, purified from hybridoma cell culture

Synonim(y):

Anti-IAA, Anti-Indole-3-acetic acid

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Rozmiar/SKUDostępnośćCena netto
200 μL
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2670,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
1E11-C11, monoclonal
Application:
DB, ELISA (i), IF, IHC, WB
Citations:
15

2670,00 zł


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biological source

mouse

Quality Segment

recombinant

expressed in mouse cell line

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1E11-C11, monoclonal

form

PBS solution

species reactivity

plant

technique(s)

dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA, western blot: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.

Immunogen

indoleacetic acid (IAA) carboxyl linked to ovalbumin.

Application

Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.
Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja
B3275A5228SAB4200787
description

clone 1E11-C11, purified from hybridoma cell culture

description

clone RG-16, purified immunoglobulin, buffered aqueous solution

description

clone 1A4, purified from hybridoma cell culture

description

clone GA-1, purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

-

clone

1E11-C11, monoclonal

clone

RG-16, monoclonal

clone

1A4, monoclonal

clone

GA-1, monoclonal

species reactivity

plant

species reactivity

rabbit

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

species reactivity

human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

dot blot: suitable, western blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA

technique(s)

western blot: 1:200,000, direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)

technique(s)

indirect ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells, indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections, immunocytochemistry: suitable using smooth muscle cells, western blot: suitable using smooth muscle cells, microarray: suitable

technique(s)

indirect ELISA: 0.06-0.12 μg/mL using 5 μg/ml secretory human IgA for coating.


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Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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