A2237

Phosphatase, Alkaline shrimp

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

• Synonim(y): Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Numer CAS: 9001-78-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numer WE: 3.1.3.1 (BRENDA, IUBMB)
• MDL number: MFCD00131849
• eCl@ss: 42010105
• Recombinant: expressed in proprietary host
• Concentration: ≥900 DEA units/mL

Właściwości

• recombinant: expressed in proprietary host
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• concentration: ≥900 DEA units/mL
• technique(s): protein detection: suitable
• foreign activity: RNAse, DNAse, none detected
• shipped in: dry ice
• storage temp.: −20°C

Opis

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl₂

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H334
• Ostrzeżenia: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 10 - Combustible liquids
• Co się dzieje?: WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable