CAPHOS

Calcium Phosphate Transfection Kit

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

• Synonim(y): Gene delivery
• UNSPSC Code: 12352200
• EC Number: 233-140-8
• NACRES: NA.85
• Form: solution
• Grade: Molecular Biology
• Technique(s): transfection: suitable

Właściwości

• grade: Molecular Biology
• Quality Segment: 200
• form: solution
• usage: kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes)
• technique(s): transfection: suitable
• shipped in: dry ice
• storage temp.: −20°C

Opis

General description

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.

Application

Calcium Phosphate Transfection Kit has been used:

• to enable transfection
• to transfect Hek293T cells
• to transfect H29D cells

Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:

BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D

Biochem/physiol Actions

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl₂ solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

Features and Benefits

• Suitable for transient and stable transfection
• Reproducible for a wide range of cell types
• Widely referenced
• Inexpensive

Other Notes

The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)

The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H319
• Ostrzeżenia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Klasa składowania: 12 - Non Combustible Liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Co się dzieje?: WGK 1