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CS0410

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

Synonim(y):

GST Enzyme Activity Kit

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Do Państwa/SKUDostępnośćCena netto
1 kit
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
3360,00 zł

Informacje o tej pozycji

UNSPSC Code:
12161503
EC Number:
202-551-4
NACRES:
NA.84

3360,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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usage

sufficient for 500 multiwell tests

Quality Segment

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... GSTA4(2941), GSTM1(2944)

General description

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Application

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.
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Ta pozycja
CS0260CS1020CGP1
Glutathione-S-Transferase (GST) Assay Kit sufficient for 500 multiwell tests

Sigma-Aldrich

CS0410

Glutathione-S-Transferase (GST) Assay Kit

Glutathione Assay Kit sufficient for 700 assays

Sigma-Aldrich

CS0260

Glutathione Assay Kit

Glutathione Assay Kit, Fluorimetric sufficient for 200 multiwell tests

Sigma-Aldrich

CS1020

Glutathione Assay Kit, Fluorimetric

Glutathione Peroxidase Cellular Activity Assay Kit Sufficient for 100 colorimetric tests

Sigma-Aldrich

CGP1

Glutathione Peroxidase Cellular Activity Assay Kit

description

sufficient for 500 multiwell tests

description

sufficient for 700 assays

description

sufficient for 200 multiwell tests

description

Sufficient for 100 colorimetric tests

Gene Information

human ... GSTA4(2941), GSTM1(2944)

Gene Information

human ... GSR(2936)

Gene Information

human ... GSTA4(2941)

Gene Information

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 500 multiwell tests

usage

sufficient for 700 assays

usage

sufficient for 200 multiwell tests

usage

sufficient for 100 tests

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Tylko elementy zestawu

Numer produktu
Opis
  • Dulbecco's Phosphate Buffered Saline 100 mL
  • L-Glutathione Reduced 1 g
  • GST (Control) .1 mL
  • Sample Buffer 5 mL
  • Substrate (CDNB) 1.2 mL

signalword

Danger

flash_point_f

Not applicable

flash_point_c

Not applicable

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

Klasa składowania

3 - Flammable liquids

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–6 of 6 Questions  

  1. Is this kit suitable to measure GST enzyme activity in plants? If yes, would plant tissue homogenate will work for this kit?

    1 answer

    1. This kit is not tested specifically for use in plant tissue. However this kit is based on the same principal as referenced in Mozer, T. J., et al., Purification and characterization of corn glutathione S-transferase. Biochemistry 22, 1068-1072 (1983) and is expected to be suitable. This kit has also been used successfully to measure GST activity in plants by other researcher. Please see the following link to review one such example:
      https://pmc.ncbi.nlm.nih.gov/articles/PMC10669765/

      Please see the following link to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Is there a protocol available for preparing tissue for assay with the CS0410 Glutathione-S-Transferase (GST) Assay Kit?

    1 answer

    1. A specific tissue preparation protocol is not included with this kit. Product C3228, CelLytic MT reagent for mammalian tissue, is suitable. Please see the link below to review the product datasheet and protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/238/c3228bul.pdf

      Flash freezing the sample in liquid nitrogen and cold homogenization methods are also suitable for use with this kit. There are a number of methods available in published literature. Please see the link below for articles that may be helpful:
      https://scholar.google.com/scholar?q=tissue+lysate+protocol&hl=en&as_sdt=0&as_vis=1&oi=scholart

      Helpful?

  5. Is this suitable for detecting all of GST activity or is there for specific GST family for example GSTA or GSTM activity only?

    1 answer

    1. This product has not been evaluated for specificity to each GST isozyme. However, there is no evidence to suggest that this kit does not react to all GST isozymes.

      Helpful?

  6. ¿Cuál es el mejor protocolo para su utilización?

    1 answer

    1. The product datasheet is listed in the DOCUMENTATION section under 'More Documents'. Please see the links below to access the Product Datasheet:
      https://www.sigmaaldrich.com/product/sigma/cs0410#product-documentation
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf

      Helpful?

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