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CS0410

Glutathion-S-Transferase(GST)-Assay-Kit

sufficient for 500 multiwell tests

Synonym(e):

GST-Enzymaktivitätskit

zur Ansicht der Organisations- und Vertragspreise.

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Ihnen/SKUVerfügbarkeitPreis
1 kit
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CHF 703.00

Über diesen Artikel

UNSPSC Code:
12161503
EC Number:
202-551-4
NACRES:
NA.84

CHF 703.00


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usage

sufficient for 500 multiwell tests

Quality Segment

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

Das Glutathion-S-Transferase(GST)-Assaykit wurde zur Messung der Gesamt-GST-Aktivität entwickelt. Es kann zum Messen der GST-Aktivität in Zell- und Bakterienlysaten, Gewebehomogenaten und Plasma-
und Erythrozytenlysaten eingesetzt werden.

Application

Das Glutathion-S-Transferase-Assaykit nutzt 1-Chlor-2,4-dinitrobenzol (CDNB), das sich für das breiteste Spektrum von GST-Isozymen eignet. Bei der Konjugation der Thiolgruppe von Glutathion an das CDNB-Substrat kommt es zu einer Steigerung der Absorption bei 340 nm.

Biochem/physiol Actions

Glutathion-S-Transferasen (GSTs) sind eine Gruppe von Enzymen, die bei der Entgiftung zahlreicher Xenobiotika in Säugern wichtig sind. Die Enzyme schützen die Zellen vor Giftstoffen, indem sie die Thiolgruppe des Glutathions an elektrophile Xenobiotika konjugieren und die Zellen auf diese Weise vor den mutagenen, karzinogenen und toxischen Wirkungen der Verbindungen schützen. Die GST-Aktivität tritt in Pflanzen, Insekten, Hefen, Bakterien und den meisten Säugergeweben auf, besonders aber in der Leber, der bei der Entgiftung eine wichtige Funktion zufällt.

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Dieser Artikel
CS0260CS1020CGP1
Gene Information

human ... GSTA4(2941), GSTM1(2944)

Gene Information

human ... GSR(2936)

Gene Information

human ... GSTA4(2941)

Gene Information

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 500 multiwell tests

usage

sufficient for 700 assays

usage

sufficient for 200 multiwell tests

usage

sufficient for 100 tests

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C


Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • Dulbecco's Phosphate Buffered Saline 100 mL

  • L-Glutathione Reduced 1 g

  • GST (Control) .1 mL

  • Sample Buffer 5 mL

  • Substrate (CDNB) 1.2 mL

signalword

Danger

flash_point_f

Not applicable

flash_point_c

Not applicable

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

Lagerklasse

3 - Flammable liquids



Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is there a protocol available for preparing tissue for assay with the CS0410 Glutathione-S-Transferase (GST) Assay Kit?

    1 answer
    1. A specific tissue preparation protocol is not included with this kit. Product C3228, CelLytic MT reagent for mammalian tissue, is suitable. Please see the link below to review the product datasheet and protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/238/c3228bul.pdf

      Flash freezing the sample in liquid nitrogen and cold homogenization methods are also suitable for use with this kit. There are a number of methods available in published literature. Please see the link below for articles that may be helpful:
      https://scholar.google.com/scholar?q=tissue+lysate+protocol&hl=en&as_sdt=0&as_vis=1&oi=scholart

      Helpful?

  4. Is this suitable for detecting all of GST activity or is there for specific GST family for example GSTA or GSTM activity only?

    1 answer
    1. This product has not been evaluated for specificity to each GST isozyme. However, there is no evidence to suggest that this kit does not react to all GST isozymes.

      Helpful?

  5. ¿Cuál es el mejor protocolo para su utilización?

    1 answer
    1. The product datasheet is listed in the DOCUMENTATION section under 'More Documents'. Please see the links below to access the Product Datasheet:
      https://www.sigmaaldrich.com/product/sigma/cs0410#product-documentation
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf

      Helpful?

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