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D4540

Dimethyl sulfoxide

≥99.5% (GC), suitable for plant cell culture

1 of 2 reviewers received a sample product or took part in a promotion

Synonim(y):

DMSO

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Rozmiar/SKUDostępnośćCena netto
100 mL

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

329,00 zł
500 mL

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

690,00 zł
1 L

Dostępne do wysyłki DZISIAJzSchnelldorf Distribution

1160,00 zł

Informacje o tej pozycji

Wzór liniowy:
(CH3)2SO
Numer CAS:
Masa cząsteczkowa:
78.13
UNSPSC Code:
12191502
NACRES:
NA.71
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.5% (GC)
Technique(s):
cell culture | plant: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

329,00 zł


Dostępne do wysyłki DZISIAJSzczegóły


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Quality Segment

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5% (GC)

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | plant: suitable, cryopreservation: suitable

impurities

<0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Application

Dimethyl sulfoxide has been used:
  • in the preparation of bisphenol A (BPA)
  • in cell proliferation assay (MTT assay)
  • in chemosensitivity assay
  • as the vehicle control in all experiments like apoptosis assay
  • in ethoxyresorufin-O-deethylation (EROD) assay

Biochem/physiol Actions

DMSO (dimethyl sulfoxide), an organic solvent possesses several pharmaceutical activities such as analgesic, diuretic, bacteriostatic, membrane-penetrant, anti-inflammatory, vasodilatory and cholinesterase inhibitory effects. It is a by-product of the lumber industry and used as an industrial solvent. DMSO also serves as a therapeutic agent to treat interstitial cystitis and arthritis.

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
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Ta pozycja
PHR1309317275D2653
description

≥99.5% (GC), suitable for plant cell culture

description

Pharmaceutical Secondary Standard; Certified Reference Material

description

ACS Reagent Grade.

description

Hybri-Max™, 0.2 μm filtered, BioReagent, suitable for hybridoma, ≥99.7%, Ampoule

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

300

assay

≥99.5% (GC)

assay

-

assay

≥99% (GC)

assay

≥99.7%

technique(s)

cell culture | plant: suitable, cryopreservation: suitable

technique(s)

gas chromatography (GC): suitable, HPLC: suitable

technique(s)

-

technique(s)

cryopreservation: suitable, cell culture | hybridoma: suitable

solubility

H2O: miscible (completely)

solubility

-

solubility

-

solubility

H2O: miscible (completely)

form

liquid

form

-

form

liquid

form

liquid

bp

189 °C (lit.)

bp

-

bp

189 °C (lit.)

bp

189 °C (lit.)


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Klasa składowania

10 - Combustible liquids

Co się dzieje?

WGK 1

Temperatura zapłonu (°F)

188.6 °F - closed cup

Temperatura zapłonu (°C)

87 °C - closed cup

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–10 of 11 Questions  
  1. Can I use this product (suitable for plant cell culture) for cyropreservation of mammalian cells? Does cryopreservation of mammalian cells require sterile-filtered DMSO?

    1 answer
    1. D4540 is listed as suitable for cryopreservation.
      However, D2650 is already pre-sterile and has documentation with a protocol for cryopreservation of human and animal cell lines.

      See these links:
      https://www.sigmaaldrich.com/product/sigma/d2650
      https://www.sigmaaldrich.com/product/sigma/d2650#product-documentation

      Look for the Product Information Sheet. Also, see this link for more information:
      https://www.sigmaaldrich.com/products/cell-culture-and-analysis/cell-culture-supplements-and-reagents/cell-freezing-reagents

      Helpful?

  2. Can I autoclave this product?

    1 answer
    1. DMSO is not autoclavable in neat form. It is highly unlikely that microorganisms would survive in this environment. Should sterilization be necessary a PTFE, Polypropylene, or Nylon filter membrane are recommended for sterile-filtration.

      Helpful?

  3. What is the DMSO dissolved in?

    1 answer
    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

      Helpful?

  4. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  5. Is Dimethyl sulfoxide a sterile product?

    1 answer
    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

  6. What concentration of DMSO do I use in a cell freezing media?

    1 answer
    1. DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

      Helpful?

  7. Does DMSO have any synthetic utility?

    1 answer
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Where does DMSO come from?

    1 answer
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  10. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer
    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      Helpful?

1–10 of 11 Questions  

Reviews

Active Filters

  1. San Diego
    • Review 1
    • Votes 8
    5 out of 5 stars.

    Works on mammalian cells

    Good for mammalian cell culture treatments of compound

    Helpful?

  2. 1 Ratings-only review