D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

• Synonim(y): DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
• Numer CAS: 9003-98-9
• UNSPSC Code: 12352204
• NACRES: NA.53
• EC Number: 232-667-0
• MDL number: MFCD00130918
• Numer WE: 3.1.21.1 (BRENDA, IUBMB)
• Specific activity: 10000 units/mg protein
• Biological source: bovine pancreas
• Concentration: 5—15 mg protein/mL

Właściwości

• biological source: bovine pancreas
• Quality Segment: 200
• grade: Molecular Biology
• form: buffered aqueous glycerol solution
• specific activity: 10000 units/mg protein
• mol wt: 29.1 kDa
• concentration: 5—15 mg protein/mL
• technique(s): DNA extraction: suitable
• input: general use sample(s)
• suitability: suitable for molecular biology
• UniProt accession no.: P00639
• application(s): cell analysis
• foreign activity: RNase, none detected
• storage temp.: −20°C
• Gene Information: cow ... DNASE1(282217)

Opis

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Other Notes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)