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D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Synonym(s):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.52

klasa czystości

for molecular biology

Poziom jakości

200

gęstość pary

2.7 (vs air)

ciśnienie pary

0.42 mmHg ( 20 °C)

linia produktu

BioReagent

próba

≥99.9%

forma

liquid

temp. samozapłonu

573 °F

okres trwałości

Recommended retest period - 2 years

granice wybuchowości

42 %, 63 °F

technique(s)

DNA sequencing: suitable
PCR: suitable
transfection: suitable

zanieczyszczenia

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

kolor

colorless

współczynnik refrakcji

n20/D 1.479 (lit.)

tw

189 °C (lit.)

mp

16-19 °C (lit.)

rozpuszczalność

H2O: miscible (completely)

gęstość

1.10 g/mL (lit.)

absorpcja

passes test

przydatność

suitable for molecular biology

obecność zanieczyszczeń

DNase and RNase, none detected

temp. przechowywania

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Opis ogólny

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Zastosowanie

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Opakowanie

50, 100, 250, 500 mL in glass bottle
1 L in glass bottle

Cechy i korzyści

This product is free from DNases, RNases, phosphatases and proteases.

Przestroga

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Kod klasy składowania

10 - Combustible liquids

WGK

WGK 1

Temperatura zapłonu °F

188.6 °F - closed cup

Temperatura zapłonu °C

87 °C - closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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