Unfortunately, this product is not tested for the specific application. A literature search is recommended for further information.
Przejdź do
D8515
Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA
lyophilized powder
Synonim(y):
DNA, Deoxyribonucleic acid, ds-DNA-Cellulose, DNA-Cellulose
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 632,00 zł |
5 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2330,00 zł |
10 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3510,00 zł |
Informacje o tej pozycji
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
632,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócbiological source
bovine (calf) thymus
Quality Segment
form
lyophilized powder
extent of labeling
3-8 mg double-stranded calf thymus DNA/g
storage temp.
−20°C
General description
Application
Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.
Analysis Note
May contain a trace of Tris and EDTA.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder | description Genomic, unsheared | description Type I, fibers | description Purified DNA from calf thymus. A260/280 nm: ≥ 1.8); S20w: 15.4-17.4; hyperchromicity: ≥27%. |
| biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form fibers | form solid |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| extent of labeling 3-8 mg double-stranded calf thymus DNA/g | extent of labeling - | extent of labeling - | extent of labeling - |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
Can it be used to evaluate nucleosides from microorganisms? Example: thermonuclease in S. aureus
1 answer-
Helpful?
-
-
Do you have recommendations for how to swell the DNA-cellulose and prepare it for subsequent use with DNA-binding proteins?
1 answer-
This product may be swelled in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
Helpful?
-
-
How to dissolve this product to coat elisa plate, please?
1 answer-
This product is not tested for the suitability with coating ELISA plates. It may be helpful to read through the peer-reviewed articles associated with this product: https://www.sigmaaldrich.com/search/d8515?focus=papers&page=1&perpage=30&sort=relevance&term=d8515&type=citation_search
Helpful?
-
-
What buffer do you use to swell the Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515?
1 answer-
DNA-Cellulose can be swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
Helpful?
-
-
Can Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515, be exposed to high salt?
1 answer-
DNA-Cellulose is stable in up to 5M NaCl. DNA-Cellulose should not be exposed to solutions of NaOH, since NaOH denatures DNA from double-stranded to single-stranded.
Helpful?
-
-
Can Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA, be used for purification of DNA-dependent RNA Polymerase?
1 answer-
We have not tested Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA for this application. However, the following references utilizes double-stranded DNA cellulose for purification of DNA-dependent RNA Polymerase:J. of Bacteriology, 172, 5624-5630 (1990)Biochem. J.,133, 201-203 (1973)
Helpful?
-
-
How do you store Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515, once it is swollen?
1 answer-
DNA-Cellulose can be stored swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.5 - 8) for several months at 4°C (without a bacteriostat).
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How do you regenerate the column using Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515?
1 answer-
A suggested procedure for the regeneration of a DNA-Cellulose column would be to use 5 - 10 column volumes of 2M NaCl in buffer, followed by the same volume of buffer alone.
Helpful?
-