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Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Numer MDL:

Poziom jakości







cell culture | mammalian: suitable


endotoxin, tested


glucose: no (Dextro)
sodium pyruvate: no



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Dulbecco′s Phosphate Buffered Saline has been used for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate. It has also been used to wash the wells of microtiter plate.
DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Kod klasy składowania

12 - Non Combustible Liquids



Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Wprowadź numer partii, aby wyszukać certyfikat analizy (COA).

Świadectwo pochodzenia

Wprowadź numer partii, aby wyszukać świadectwo pochodzenia (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the formulation for Product D8537, Dulbecco's Phosphate?

    The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.    

  4. Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

    This is a 1X concentration. The 10X concentration is listed under product D1408.

  5. What is the pH of a solution of Product D8537, Dulbecco's Phosphate?

    The pH will be in the range of 7.1-7.5, according to the specification sheet.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. What is a Salt Toxicity Test?

    The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted.  They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour.  The cells are counted and %viability determined.  The cells are then cultured for an additional 7 hours (total of 8 hours).  The cells are counted again and the % viability determined.  The viability at 1 hr has to be >95% of the original viability.  The viability at 8 hrs must be >75% of the original viability.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Arturo Levican et al.
Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
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Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
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Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Saabah B Mahbub et al.
Scientific reports, 7(1), 15792-15792 (2017-11-19)
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Biological procedures online, 20, 20-20 (2018-10-26)
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