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F6008

Ficin from fig tree latex

lyophilized powder

Synonim(y):

Debricin, Ficain, higueroxyl delabarre

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Do Państwa/SKUDostępnośćCena netto
100 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1020,00 zł
200 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1890,00 zł
1000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
7110,00 zł

Informacje o tej pozycji

Numer CAS:
9001-33-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-599-1
MDL number:
MFCD00081597
Numer WE:
3.4.22.3 (BRENDA, IUBMB)
Specific activity:
≥1.0 units/mg protein
Biological source:
fig tree (latex)

1020,00 zł

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biological source

fig tree (latex)

Quality Segment

300

form

lyophilized powder

specific activity

≥1.0 units/mg protein

mol wt

23.8 kDa

composition

Protein, 80-100% biuret

storage temp.

−20°C

General description

Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Application

Ficin from fig tree latex has been used:
  • in the proteolysis of tendon collagen
  • in the digestion of Chlorophyta Ulva rigida
  • to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure.

Physical form

Lyophilized powder containing L-cysteine and trace EDTA

Preparation Note

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

Other Notes

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
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F4165P3375F4125
Ficin from fig tree latex lyophilized powder

Sigma-Aldrich

F6008

Ficin from fig tree latex

Ficin from fig tree latex powder, ≥0.1 unit/mg solid

Sigma-Aldrich

F4165

Ficin from fig tree latex

Papain from papaya latex crude powder, 1.5-10 units/mg solid

Sigma-Aldrich

P3375

Papain from papaya latex

Ficin from fig tree latex saline suspension, ≥1.0 units/mg protein (biuret)

Sigma-Aldrich

F4125

Ficin from fig tree latex

description

lyophilized powder

description

powder, ≥0.1 unit/mg solid

description

crude powder, 1.5-10 units/mg solid

description

saline suspension, ≥1.0 units/mg protein (biuret)

biological source

fig tree (latex)

biological source

fig tree (latex)

biological source

-

biological source

fig tree (latex)

specific activity

≥1.0 units/mg protein

specific activity

≥0.1 unit/mg solid

specific activity

1.5-10 units/mg solid

specific activity

≥1.0 units/mg protein (biuret)

form

lyophilized powder

form

powder

form

crude powder

form

saline suspension

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.4 kDa

mol wt

23.8 kDa

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Lot/Batch Number

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Questions

  1. What is this used for exactly? In lay terms please.

    1 answer

    1. Ficin is an enzyme that has a number of uses in several markets. As mentioned in the DESCRIPTION section of the product detail page, ficin from fig tree latex has been used:

      in the proteolysis of tendon collagen (tendons are softened in meat/muscles)
      in the digestion of Chlorophyta Ulva rigida (softens this algae which is used in 'sea salad' and animal feed)
      to investigate it's effect on protease-activated receptors 2 and 4 activation to induce itch in skin (studies indicate it can cause itching)
      in disrupting the staphylococcal biofilms, making it accessible antibiotics exposure. (Staph can be very resistant to antibiotics, particularly in 'biofilms'. Ficin digests or breaks down this film so that antibiotics are more effective.)

      It is also used in the food industry, primarily dairy products and is useful in clotting milk to aid in cheese production.
      Ficin has several medical uses, as well. This product is for research use only, however the link below describes some of the medical applications of Ficin:
      https://www.webmd.com/vitamins/ai/ingredientmono-141/ficin

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