This product, fluorescein isothiocyanate, is used for protein and antibody conjugation followed by fluorescence analysis. However, this product has not been validated as a starch-specific stain for microscopy. A literature search did not identify publications supporting its use as a fluorescent starch stain for confocal laser scanning microscopy. Therefore, a concentration and solvent cannot be recommended for this application.
Przejdź do
F7250
Fluorescein isothiocyanate isomer I
≥90% purity (HPLC), powder
Synonim(y):
FITC, Fluorescein 5-isothiocyanate
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Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C21H11NO5S
Numer CAS:
Masa cząsteczkowa:
389.38
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
222-042-0
MDL number:
Beilstein/REAXYS Number:
1407295
eCl@ss:
32039090
Informacje o cenach i dostępności nie są obecnie dostępne.
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Pozwól nam pomócNazwa produktu
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
Quality Segment
assay
≥90% (HPLC)
form
powder
technique(s)
titration: suitable
color
orange to dark orange
mp
>360 °C (lit.)
solubility
acetone: 1 mg/mL
fluorescence
λex 492 nm; λem 518 nm (green)
suitability
suitable for protein labeling
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
General description
Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
Application
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
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Ta pozycja | |
|---|---|
| description suitable for protein labeling, ≥90% (HPLC), powder | description ≥97.5% (HPLC) |
| technique(s) titration: suitable | technique(s) titration: suitable |
| suitability suitable for protein labeling | suitability - |
| color orange to dark orange | color yellow to very dark yellow |
| fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) |
| form powder | form - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Klasa składowania
11 - Combustible Solids
WGK
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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We're developing wheat/rice flour + pea protein yogurts and want to image them by CLSM. Is this FITC suitable for fluorescent starch staining? What concentration and solvent do you recommend?
1 answer-
Helpful?
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Are cell membranes of living cells permeable for FITC?
1 answer-
FITC is not membrane permeable in healthy cells, it binds to surface proteins. Conjugates such as FITC-Dextran is an example of a more cell-permeable option.
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Is Product No. F7250, Fluorescein isothiocyanate isomer I, pharmaceutical grade?
1 answer-
No. The product is considered suitable for research use only.
Helpful?
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How stable is FITC Isomer 1 (e.g., Product No. F7250) once conjugated to an antibody?
1 answer-
FITC can photo bleach fairly rapidly. The prepared solution should be stored in a dark container and protected from light. When viewing the antibody staining under a microscope, it is advisable to use subdued fluorescent light when possible. If the fluorescent light stays at one position for too long, photo bleaching is noticeable.
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After the FITC (e.g., Product No. F7250) is conjugated to the protein, what is the solution stability of the conjugated protein?
1 answer-
The stability of the conjugated protein will be essentially the stability of the unconjugated protein. Some conjugated proteins might be stable when stored at -20°C and other conjugated proteins will have better solution stability at 2-8°C If the protein concentration is too low, this also has a negative effect upon the stability of the conjugated protein.
Helpful?
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Is the intensity of FITC (e.g., Product No. F7250) pH dependent?
1 answer-
Yes; many articles have reported the pH dependence of the fluorescence of fluorescein derivatives. See the following references: Archives Biochem. Biophys., 133, 263 (1969); J. Immunol., 95, 1165 (1966); Archives Biochem. Biophys., 73, 1 (1958); Standard test for labeling efficiency of FITC with BSA: NCCLS Approved Standard: ASM-1, 2nd ed., June, 1975; Characterization of FITC-dyes by IR and TLC: Analytical Biochem., 29, 526 (1969); Fluorescent labeling of proteins: Proc. Nat. Acad. Sci., 72, 459 (1975); Amer. J. Pathol., 34, 1081 (1958); Fluorescent labeling of a lectin: Meth. Enzymol., 81, 65 (1982).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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