The GALS kit, identified by product number F1797, is not tested or recommended for use with a mouse brain that has been fixed using a different fixative for 4 days or longer. The standard kit procedure fixes cells for only 10 minutes, whereas the fixation period used for the mouse brain is 396 times longer than what is recommended in the kit protocol. The kit was never tested for use on a mouse brain fixed using another fixative. Therefore, it is uncertain whether the fixation used for the mouse brain might produce acceptable results
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GALS
β-Galactosidase Reporter Gene Staining Kit
Synonim(y):
β-galactosidase, LacZ
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1520,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12161503
Storage temp.:
−20°C
Shipped in:
dry ice
1520,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócusage
kit sufficient for 100 tests (using a 3.5 cm dish)
Quality Segment
technique(s)
microbe id | staining: suitable
shipped in
dry ice
storage temp.
−20°C
General description
β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Application
β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
- sagittal brain sections in histoenzymology
- pancreas or colon sections in β-galactosidase reporter gene assay
- mice E10.5 -11.5 embryo and embryo sections
Other Notes
The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.
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Ta pozycja | |||
|---|---|---|---|
| description - | description - | description clone GAL-13, ascites fluid | description clone GAL-40, ascites fluid |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) immunocytochemistry: suitable, dot blot: suitable using soluble β-galactosidase, indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase | technique(s) western blot: 1:1,000 using denatured-reduced Escherichia coli β-galactosidase, dot blot: suitable using soluble β-galactosidase, immunocytochemistry: suitable |
| usage kit sufficient for 100 tests (using a 3.5 cm dish) | usage kit sufficient for 100 histochemical assays | usage - | usage - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Słowo ostrzegawcze
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Narządy docelowe
Respiratory system
supp_hazards
Klasa składowania
3 - Flammable liquids
Temperatura zapłonu (°F)
132.8 °F
Temperatura zapłonu (°C)
56 °C
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Is the GALS kit compatible with fixed, frozen mouse brain that used a totally different fixative for 4 days or longer?
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