L3771

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

• Synonim(y): Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Wzór liniowy: CH₃(CH₂)₁₁OSO₃Na
• Numer CAS: 151-21-3
• Masa cząsteczkowa: 288.38
• Beilstein: 3599286
• Numer WE: 205-788-1
• Numer MDL: MFCD00036175
• Kod UNSPSC: 12161902
• eCl@ss: 39093306
• Identyfikator substancji w PubChem: 24896351
• NACRES: NB.22

Właściwości

• pochodzenie biologiczne: synthetic
• Poziom jakości: 200
• klasa czystości: for molecular biology
• opis: anionic
• linia produktu: BioReagent
• Próba: ≥98.5% (GC)
• Formularz: powder
• masa cząsteczkowa: micellar 18,000
• liczba agregacji: 62
• metody: electrophoresis: suitable
• zanieczyszczenia: ≤2.0% water (Karl Fischer)
• CMC: 7-10 mM (20-25°C)
• mp: 204-207 °C (lit.)
• temp. przejścia: cloud point >100 °C
• rozpuszczalność: water: soluble
• gęstość: 1.03 g/cm³ at 20 °C (68 °F)
• ślady anionów: chloride (Cl^-): ≤500 ppm; phosphate (PO₄^3-): ≤10 ppm
• ślady kationów: heavy metals (as Pb): ≤10 ppm
• Absorpcja: ≤0.1 at 260 at 3%; ≤0.1 at 280 at 3%
• HLB: 40
• przydatność: suitable for electrophoresis
• obecność zanieczyszczeń: DNase, RNase, none detected
• temp. przechowywania: room temp
• ciąg SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• Klucz InChI: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Opis

Opis ogólny

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is an anionic surfactant widely used in various scientific applications, particularly in molecular biology, biochemical, and cell biology research. Its effectiveness stems from its amphiphilic nature, allowing it to interact with both hydrophobic and hydrophilic molecules. SDS is a versatile detergent that excels in solubilizing and denaturing proteins, making it an essential tool in protein extraction and purification procedures. By disrupting protein structures, SDS facilitates the separation of proteins based on their molecular weight during SDS-PAGE electrophoresis.

Beyond protein analysis, SDS also finds applications in the electrophoretic separation of lipids, enabling the study and characterization of these important cellular components. Additionally, SDS is employed in various hybridization and molecular biology techniques, demonstrating its versatility across a range of research areas. SDS′s effectiveness in both ionic and anionic conditions further expands its utility, allowing it to adapt to diverse experimental setups and requirements. Its ability to unravel proteins by binding to peptide chains and imparting a negative charge effectively eliminates protein shape as a factor for gel separation, ensuring accurate protein size determination.

Zastosowanie

Anionic detergent

Sodium dodecyl sulfate has been used:

• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Cechy i korzyści

• Highly versatile surfactant for your molecular biology and biochemical research
• Suitable for electrophoresis applications
• Tested to confirm low levels of heavy metal contamination,ensuring suitability for various applications.
  

Komentarz do analizy

Tested for use in denatured polyacrylamide gel electrophoresis.

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H228,H302 + H332,H315,H318,H335,H412
• Zwroty wskazujące środki ostrożności: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 4.1B - Flammable solid hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): 338.0 °F
• Temperatura zapłonu (°C): 170 °C
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges