Merck
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L3771

Sigma-Aldrich

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Synonym(s):
Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
Beilstein:
3599286
Numer EC:
Numer MDL:
eCl@ss:
39093306
Identyfikator substancji w PubChem:
NACRES:
NB.22

klasa czystości

for molecular biology

Poziom jakości

200

opis

anionic

linia produktu

BioReagent

próba

≥98.5% (GC)

masa cząsteczkowa

micellar 18,000

liczba agregacji

62

technique(s)

electrophoresis: suitable

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

temp. przejścia

cloud point >100 °C

rozpuszczalność

water: soluble

ślady anionów

chloride (Cl-): ≤500 ppm
phosphate (PO43-): ≤10 ppm

ślady kationów

heavy metals (as Pb): ≤10 ppm

absorpcja

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

40

przydatność

suitable for electrophoresis

obecność zanieczyszczeń

DNase, RNase, none detected

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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Opis ogólny

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Zastosowanie

Sodium dodecyl sulfate has been used:
  • in chromatin immunoprecipitation
  • in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
  • as a component of radioimmunoprecipitation assay buffer for immunoblotting
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.
Anionic detergent

Opakowanie

25, 100, 500 g in poly bottle
1 kg in poly bottle

Komentarz do analizy

Tested for use in denatured polyacrylamide gel electrophoresis.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

4.1B - Flammable solid hazardous materials

WGK

WGK 2

Temperatura zapłonu °F

338.0 °F

Temperatura zapłonu °C

170 °C

Środki ochrony indywidualnej

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certificate of Analysis

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Certificate of Origin

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