Merck

L3771

Sigma-Aldrich

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

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Synonim(y):
Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
Wzór liniowy:
CH3(CH2)11OSO3Na
Numer CAS:
Masa cząsteczkowa:
288.38
Beilstein:
3599286
Numer EC:
Numer MDL:
eCl@ss:
39093306
Identyfikator substancji w PubChem:
NACRES:
NB.22

pochodzenie biologiczne

synthetic

Poziom jakości

klasa czystości

for molecular biology

opis

anionic

linia produktu

BioReagent

Próba

≥98.5% (GC)

forma

powder

masa cząsteczkowa

micellar 18,000

liczba agregacji

62

technique(s)

electrophoresis: suitable

zanieczyszczenia

≤2.0% water (Karl Fischer)

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

temp. przejścia

cloud point >100 °C

rozpuszczalność

water: soluble

gęstość

1.03 g/cm3 at 20 °C (68 °F)

ślady anionów

chloride (Cl-): ≤500 ppm
phosphate (PO43-): ≤10 ppm

ślady kationów

heavy metals (as Pb): ≤10 ppm

absorpcja

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

40

przydatność

suitable for electrophoresis

obecność zanieczyszczeń

DNase, RNase, none detected

temp. przechowywania

room temp

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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Ta pozycja
71725L6026RDD021
Sodium dodecyl sulfate BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Sigma-Aldrich

L3771

Sodium dodecyl sulfate

Essential+ Grade
Sodium dodecyl sulfate BioUltra, for molecular biology, ≥99.0% (GC)

Sigma-Aldrich

71725

Sodium dodecyl sulfate

Premium Grade
Sodium dodecyl sulfate BioXtra, ≥99.0% (GC)

Sigma-Aldrich

L6026

Sodium dodecyl sulfate

Premium Grade
Sodium dodecyl sulfate anhydrous, free-flowing, Redi-Dri™, ACS reagent, ≥99.0%

Sigma-Aldrich

RDD021

Sodium dodecyl sulfate

Essential Grade
description

anionic

description

anionic

description

anionic

description

anionic

product line

BioReagent

product line

BioUltra

product line

BioXtra

product line

Redi-Dri

assay

≥98.5% (GC)

assay

≥99.0% (GC)

assay

≥99.0% (GC)

assay

≥99.0%

mol wt

micellar 18,000

mol wt

micellar avg mol wt 18,000

mol wt

micellar avg mol wt 18,000

mol wt

288.38 g/mol

aggregation number

62

aggregation number

62

aggregation number

62

aggregation number

-

Opis ogólny

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is an anionic surfactant widely used in various scientific applications, particularly in molecular biology, biochemical, and cell biology research. Its effectiveness stems from its amphiphilic nature, allowing it to interact with both hydrophobic and hydrophilic molecules. SDS is a versatile detergent that excels in solubilizing and denaturing proteins, making it an essential tool in protein extraction and purification procedures. By disrupting protein structures, SDS facilitates the separation of proteins based on their molecular weight during SDS-PAGE electrophoresis.

Beyond protein analysis, SDS also finds applications in the electrophoretic separation of lipids, enabling the study and characterization of these important cellular components. Additionally, SDS is employed in various hybridization and molecular biology techniques, demonstrating its versatility across a range of research areas. SDS′s effectiveness in both ionic and anionic conditions further expands its utility, allowing it to adapt to diverse experimental setups and requirements. Its ability to unravel proteins by binding to peptide chains and imparting a negative charge effectively eliminates protein shape as a factor for gel separation, ensuring accurate protein size determination.

Zastosowanie

Sodium dodecyl sulfate has been used:
  • in chromatin immunoprecipitation
  • in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
  • as a component of radioimmunoprecipitation assay buffer for immunoblotting
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.
Anionic detergent

Cechy i korzyści

  • Highly versatile surfactant for your molecular biology and biochemical research
  • Suitable for electrophoresis applications
  • Tested to confirm low levels of heavy metal contamination,ensuring suitability for various applications.

Komentarz do analizy

Tested for use in denatured polyacrylamide gel electrophoresis.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

4.1B - Flammable solid hazardous materials

WGK

WGK 2

Temperatura zapłonu °F

338.0 °F

Temperatura zapłonu °C

170 °C

Środki ochrony indywidualnej

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


Certificates of Analysis (COA)

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Przykład

T1503
Numer produktu
-
25G
Wielkość opakowania/ilość

Przykłady dodatkowe:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

wpisz jako 1.000309185)

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  • Jeśli znajdziesz numer partii taki jak TO09019TO, wprowadź numer partii 09019TO bez pierwszych dwóch liter.

  • Jeśli znajdziesz numer partii z kodem wypełnienia takim jak 05427ES-021, wprowadź numer partii 05427ES bez kodu wypełnienia -021.

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Protokoły

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

Powiązane treści

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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