The image below is stained with Brilliant Blue R-250
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L4631
Lysozyme from chicken egg white
For use as a marker in SDS-PAGE
Synonim(y):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 vial | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 513,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
Numer WE:
Biological source:
chicken egg white
513,00 zł
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Pomoc techniczna
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Pozwól nam pomócbiological source
chicken egg white
Quality Segment
form
powder
mol wt
single-chain 14.3 kDa
packaging
vial of 25 mg
input
bacteria and yeast sample(s)
UniProt accession no.
storage temp.
2-8°C
Gene Information
chicken ... LYZ(396218)
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
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Ta pozycja | |||
|---|---|---|---|
| description For use as a marker in SDS-PAGE | description aseptically filled | description 10 mg/mL | description dialyzed, lyophilized, powder, ~100000 U/mg |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) |
| biological source chicken egg white | biological source - | biological source chicken egg white | biological source chicken egg white |
| form powder | form powder | form liquid | form powder |
| mol wt single-chain 14.3 kDa | mol wt single-chain 14.3 kDa | mol wt single-chain 14.3 kDa | mol wt single-chain 14.3 kDa, Mr ~14600 |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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Do you have a picture of this product on a coomassie stained SDS-PAGE?
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