Catalog numbers L1668 and L8902 are suitable for cell culture assays and cell stimulation. The end user needs to design and optimize the assays. These products have not been specifically tested for intracellular cytokine staining assays. It is recommended to review the literature for further information.
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L8629
Lectin from Phaseolus vulgaris (red kidney bean)
Erythroagglutinin PHA-E
Synonim(y):
Phaseolus vulgaris agglutinin, PHA
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
5 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 984,00 zł 836,40 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352202
836,40 zł
Cena katalogowa984,00 złZaoszczędź 15%Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócQuality Segment
form
salt-free, lyophilized powder
potency
<10 μg per mL agglutination activity (with erythryocytes)
composition
Protein, ≥80% biuret
storage temp.
2-8°C
Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.
Tested for leucoagglutination.
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Ta pozycja | |||
|---|---|---|---|
| description Erythroagglutinin PHA-E | description Phytohemagglutinin PHA-M, lyophilized powder | description Phytohemagglutinin PHA-P, lyophilized powder | description BioXtra, Phytohemagglutinin PHA-P, purified by affinity chromatography, lyophilized powder |
| form salt-free, lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| potency <10 μg per mL agglutination activity (with erythryocytes) | potency <40 μg per mL agglutination activity | potency ≤10 μg/mL mitogenic activity, ≤62.5 μg per mL agglutination activity | potency ≤17 μg per mL agglutination activity |
| composition Protein, ≥80% biuret | composition Protein, 50-70% biuret | composition - | composition - |
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Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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Which PHA is more suitable for cell stimulation and intracellular cytokine staining assays?
1 answer-
Helpful?
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What do the designations -P, -L, -M, and -E represent in the names of PHA products?
1 answer-
Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:
1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.
2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.
PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.
Helpful?
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