The stability of this product after reconstitution has not been tested. It is recommended to prepare this product on an as-needed basis.
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L9656
Lipoprotein Lipase from Burkholderia sp.
lyophilized powder, ≥50,000 units/mg solid
Synonim(y):
Diacylglycerol acylhydrolase, Diacylglycerol lipase, Lipoprotein Lipase from Pseudomonas sp.
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
10 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1120,00 zł |
50 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4420,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-669-1
MDL number:
Specific activity:
≥50,000 units/mg solid
Biological source:
Burkholderia spp.
1120,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
Burkholderia spp.
Quality Segment
form
lyophilized powder
specific activity
≥50,000 units/mg solid
storage temp.
−20°C
General description
Lipoprotein lipase hydrolyzes triglycerides in plasma lipoproteins causing release of fatty acids for metabolic purposes in muscles and adipose tissue.
Lipoprotein Lipase (LPL) is a glycerol ester hydrolase. Several bacteria that produce LPL, belongs to the genus Pseudomonas, Serratia and Mucor.
Application
Lipoprotein Lipase from Burkholderia sp. has been used in the subcellular fractionation of mussel Mytilus galloprovincialis to identify the biomolecule attached to cytosolic fraction of okodaic acid. It has also been used to test its effect on serum amyloid A induced granulocyte colony-stimulated factor (G-CSF) expression in response to bacterial lipoprotein.LipoproteinLipase from Burkholderia sp. is suitable for the synthesis ofdiarylmethanols, a precursor for the synthesis of pharmaceutical compounds.
Biochem/physiol Actions
Due to its lipolytic activity, lipoprotein lipase was shown to effectively block the spread of hepatitis C virus into healthy cells.
Lipoprotein lipase belongs to the family of triglyceride lipases. It hydrolyses triglycerides in triglyceride-rich ApoB-containing lipoproteins.
Lipoprotein Lipase (LPL) hydrolysis triacylglycerol moieties in chylomicron and low density lipoproteins. LPL attaches lipoprotein to the vessel wall and facilitates their uptake. Abnormalities in LPL is associated with Alzheimer′s disease, atherosclerosis, obesity and chylomicronaemia.
Other Notes
One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 °C using p-nitrophenyl butyrate as substrate.
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Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder, ≥50,000 units/mg solid | description lyophilized, powder, ≥1200 U/mg | description Type XIII, lyophilized powder, ≥15 units/mg solid | description lyophilized powder, ≥4,000 units/mg solid (using olive oil) |
| biological source Burkholderia spp. | biological source bacterial (Pseudomonas spp.) | biological source - | biological source - |
| specific activity ≥50,000 units/mg solid | specific activity ≥1200 U/mg | specific activity ≥15 units/mg solid | specific activity ≥4,000 units/mg solid (using olive oil) |
| form lyophilized powder | form powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Should this product be reconstituted and frozen in aliquots, or made on as-needed basis?
1 answer-
Helpful?
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What's the molecular weight of Lipase from Burkholderia sp. (L9656)?
1 answer-
The molecular weight of this enzyme is approximately 33,000 daltons by gel filtration.
Helpful?
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what does the best reconstitute solvent?
1 answer-
This product should be reconstituted at 1 mg/ml in 100 sodium phosphate buffer, pH 7.2. Please see the Product Datasheet below, located in the 'DOCUMENTATION' section under 'More Documents':
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/149/457/l9656enz.pdfHelpful?
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