The kit instructions are published in the product datasheet. This document is located in the 'DOCUMENTATION' section under 'More Documents'. Please use the links below to access the datasheet and the documentation section of the product page:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/138/239/mak137bul.pdf
https://www.sigmaaldrich.com/product/sigma/mak137#product-documentation
Przejdź do
MAK137
Phospholipase D Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonim(y):
PLD Activity Assay Kit
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1810,00 zł |
Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
12161503
1810,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócusage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164)
mouse ... PLD1(18805), PLD2(18806), PLD3(18807), PLD4(104759), PLD5(319455), PLD6(194908)
rat ... PLD1(25096), PLD2(25097), PLD3(361527), PLD4(362792), PLD5(289270), PLD6(287366)
General description
Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.
Application
Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.
Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.
Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Biochem/physiol Actions
In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).
Features and Benefits
Compatible with high-throughput handling systems.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description sufficient for 100 colorimetric or fluorometric tests | description sufficient for 100 colorimetric tests | description Supplied by Roar Biomedical, Inc. | description sufficient for 100 fluorometric tests |
| Gene Information human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164) | Gene Information - | Gene Information human ... LPL(4023) | Gene Information human ... MAOA(4128), MAOB(4129) |
| detection method fluorometric, colorimetric | detection method colorimetric | detection method fluorometric | detection method fluorometric |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| relevant disease(s) cancer | relevant disease(s) genitourinary disorders; gastrointestinal diseases; cancer | relevant disease(s) gastrointestinal diseases; cardiovascular diseases | relevant disease(s) neurological disorders |
| usage sufficient for 100 colorimetric or fluorometric tests | usage - | usage sufficient for 100 fluorometric tests | usage sufficient for 100 fluorometric tests |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
Klasa składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
188.6 °F - closed cup
Temperatura zapłonu (°C)
87 °C - closed cup
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
Where are the Instructions for use, please?
1 answer-
Helpful?
-
-
Where are the instructions for the use of the Phospholipase D assay kit, please?
1 answer-
The Protocol for this kit is available in the product datasheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/138/239/mak137bul.pdf
Helpful?
-
-
Can the Phospholipase D Activity Assay Kit and the PLC Activity Kits be used in Human whole blood? Thanks,
1 answer-
The Phospholipase D Activity Assay Kit is suitable for use with biological samples, however whole blood has not been tested. As this is a colorimetric reaction, the resulting pink color would very likely be masked. Serum or plasma would be recommended.
Helpful?
-