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N6514

Neuraminidase from Vibrio cholerae

Name: Neuraminidase from <I>Vibrio cholerae</I>
Brand: SIGMA

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

Synonim(y):

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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Informacje o tej pozycji

Numer CAS:

9001-67-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-624-6

MDL number:

MFCD00131711

Numer WE:

3.2.1.18 (BRENDA, IUBMB)

Specific activity:

8-24 units/mg protein (Lowry, using NAN-lactose)

Biological source:

Vibrio cholerae

Informacje o cenach i dostępności nie są obecnie dostępne.

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Właściwości

biological source

Vibrio cholerae

Quality Segment

200

type

Type II

form

buffered aqueous solution

specific activity

8-24 units/mg protein (Lowry, using NAN-lactose)

foreign activity

Protease and NAN-aldolase, present

storage temp.

2-8°C

Opis

General description

Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.

Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

Application

Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Physical form

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 μm filtered..

Preparation Note

A further purification by affinity chromatography of our Type III.

Analysis Note

Preservative free.

Other Notes

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

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Ta pozycja	N7885	72197	C8052
Sigma-Aldrich N6514 Neuraminidase from Vibrio cholerae	Sigma-Aldrich N7885 Neuraminidase from Vibrio cholerae	Sigma-Aldrich 72197 Neuraminidase from Vibrio cholerae	Sigma-Aldrich C8052 Cholera Toxin from Vibrio cholerae
description Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)	description Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)	description ≥1.5 U/mL, specific activity ≥ 1.5U/mg protein	description ≥90% (SDS-PAGE), lyophilized powder
specific activity 8-24 units/mg protein (Lowry, using NAN-lactose)	specific activity 1-5 units/mg protein (Lowry, using NAN-lactose)	specific activity ≥1.5 U/mg protein	specific activity -
biological source Vibrio cholerae	biological source -	biological source Vibrio cholerae	biological source -
form buffered aqueous solution	form buffered aqueous solution	form liquid	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 300	Quality Level -	Quality Level 300
foreign activity Protease and NAN-aldolase, present	foreign activity Protease and NAN-aldolase, present	foreign activity -	foreign activity -

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Piktogramy

GHS08

Słowo ostrzegawcze

Danger

Kody zagrożeń

H334

Ostrzeżenia

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Klasa składowania

10 - Combustible liquids

Co się dzieje?

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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