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N7271

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

Synonim(y):

Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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Rozmiar/SKUDostępnośćCena netto
0.2 unit
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4910,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Biological source:
Streptococcus pneumoniae
Concentration:
≥5 units/mL

4910,00 zł


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biological source

Streptococcus pneumoniae

Quality Segment

form

buffered aqueous solution

concentration

≥5 units/mL

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Gene Information

Streptococcus pneumoniae R6 ... nanB(933504)

Biochem/physiol Actions

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Preparation Note

Expressed in glycosidase-free hosts.

Other Notes

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
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Ta pozycja
N8271N788572197
description

buffered aqueous solution

description

recombinant, expressed in E. coli, buffered aqueous solution

description

Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

description

≥1.5 U/mL, specific activity ≥ 1.5U/mg protein

biological source

Streptococcus pneumoniae

biological source

-

biological source

-

biological source

Vibrio cholerae

Gene Information

Streptococcus pneumoniae R6 ... nanB(933504)

Gene Information

-

Gene Information

-

Gene Information

-

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

liquid

concentration

≥5 units/mL

concentration

-

concentration

-

concentration

≥1.5 U/mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Klasa składowania

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–5 of 5 Questions  
  1. What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.

      Helpful?

  4. For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?

    1 answer
    1. The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.

      Helpful?

  5. What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.

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