P0389

Penicillinase from Bacillus cereus

lyophilized powder, ≥1500.00 units/mg protein (using benzylpenicillin)

• Synonim(y): β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
• Numer CAS: 9073-60-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numer WE: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• Specific activity: ≥1500.00 units/mg protein (using benzylpenicillin)
• Biological source: Bacillus sp. (Bacillus cereus)

Właściwości

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥1500.00 units/mg protein (using benzylpenicillin)
• composition: Protein, ~10%
• technique(s): activity assay: suitable
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: 2-8°C

Opis

General description

Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis.

Application

Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions.

Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.

Used in the production of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Physical form

Lyophilized powder containing phosphate and citrate buffer salts

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H317,H334
• Ostrzeżenia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Faceshields, Gloves