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P4252

Phosphatase, Alkaline from Escherichia coli

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Synonim(y):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Rozmiar/SKUDostępnośćCena netto
100 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2070,00 zł
200 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
5760,00 zł
1000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
8140,00 zł
6919,00 zł

Informacje o tej pozycji

Numer CAS:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
MFCD00131849
Numer WE:
3.1.3.1 (BRENDA, IUBMB)
Specific activity:
30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Biological source:
Escherichia coli

2070,00 zł

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biological source

Escherichia coli

Quality Segment

form

ammonium sulfate suspension

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

technique(s)

protein detection: suitable

storage temp.

2-8°C

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.

Biochem/physiol Actions

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Suspension in 2.5 M (NH4)2SO4

Analysis Note

The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.
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Ta pozycja
P5931P4069P4439
Phosphatase, Alkaline from Escherichia coli ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Sigma-Aldrich

P4252

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from Escherichia coli lyophilized powder, 30-60 units/mg protein (in glycine buffer)

Sigma-Aldrich

P5931

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from Escherichia coli buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Sigma-Aldrich

P4069

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from porcine kidney lyophilized powder, ≥100 DEA units/mg protein

Sigma-Aldrich

P4439

Phosphatase, Alkaline from porcine kidney

description

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

description

lyophilized powder, 30-60 units/mg protein (in glycine buffer)

description

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

description

lyophilized powder, ≥100 DEA units/mg protein

biological source

Escherichia coli

biological source

-

biological source

Escherichia coli

biological source

-

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

specific activity

30-60 units/mg protein (in glycine buffer)

specific activity

20-50 units/mg protein (in glycine buffer)

specific activity

≥100 DEA units/mg protein

form

ammonium sulfate suspension

form

lyophilized powder

form

buffered aqueous glycerol solution

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

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Piktogramy

Health hazard
GHS08

Słowo ostrzegawcze

Danger

Kody zagrożeń

H334

Ostrzeżenia

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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