P5442

Active Recombinant Human Phosphoglucose Isomerase

Lyophilized powder, ≥ 100U/mg protein

• Synonim(y): D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase, GPI
• UNSPSC Code: 12352204
• Form: lyophilized powder
• Assay: ≥90% (SDS-PAGE)
• Biological source: human
• Mol wt: ~63 kDa

Właściwości

• biological source: human
• Quality Segment: 100
• assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥100 U/mg Protein, ≥50 U/mg Solid
• mol wt: ~63 kDa
• suitability: suitable for molecular biology
• UniProt accession no.: P06744
• shipped in: wet ice
• storage temp.: -10 to -25°C
• Gene Information: human ... GPI(2821)

Opis

General description

PGI is a cytosolic enzyme that belongs to the isomerase class and catalyzes the reversible interconversion of glucose-6-phosphate and fructose-6-phosphate. It plays a central role in both glycolysis and gluconeogenesis. In addition to its intracellular metabolic function, PGI is also known to function extracellularly as a cytokine-like protein, including roles as autocrine motility factor (AMF) and neuroleukin, highlighting its multifunctional (“moonlighting”) properties.

Application

Phosphoglucose isomerase has been used in:

• Glycolysis and metabolic pathway studies
• Enzyme kinetics and activity assays
• Cancer metabolism and cell migration studies
• Functional studies of metabolic enzymes

Biochem/physiol Actions

PGI catalyzes the isomerization of glucose-6-phosphate to fructose-6-phosphate, a critical step in glycolysis. In addition to its metabolic role, it has extracellular signaling functions associated with tumor cell motility and growth regulation.

Physical form

Centrifuge the vial at 4ºC before opening to recover the entire contents. It is recommended that sterile water be added to the vial to prepare a stock solution.

Lyophilized from 10mM KPB (pH 8.0), 0.150M NaCl

Preparation Note

Centrifuge the vial at 4ºC before opening to recover the entire contents. It is recommended that sterile water be added to the vial to prepare a stock solution.

Unit definition: One enzyme activity unit is defined as the amount of enzyme required to convert 1.0 μmol of D-fructose-6-phosphate to D-glucose-6-phosphate per minute at pH 7.4 and a temperature of 25 °C.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H334
• Ostrzeżenia: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids