This product contains four phosphatase inhibitors. For use, one mL of cocktail solution is used to prepare 100 mL of supernatant that contains a maximum of 500 mg of protein. This results in a 1:100 dilution.
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P5726
Phosphatase Inhibitor Cocktail 2
aqueous solution (dark coloration may develop upon storage, which does not affect the activity), for the inhibition of tyrosine protein phosphatases, acid and alkaline phosphatases
Synonim(y):
Protease inhibitor
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 590,00 zł |
5 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1940,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
Solubility:
water: soluble
Storage temp.:
2-8°C
590,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócNazwa produktu
Phosphatase Inhibitor Cocktail 2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
form
aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
Quality Segment
input
general use sample(s)
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
General description
Phosphatase Inhibitor Cocktail 2 is a mixture of components designed to inhibit enzymes that modify proteins present in cell extracts, leading to improved yields of intact proteins. It has been optimized and tested for use on various animal tissues and cytosolic extracts.
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.
Features and Benefits
This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Preparation Note
This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.
Disclaimer
As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.
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Ta pozycja | |||
|---|---|---|---|
| description aqueous solution (dark coloration may develop upon storage, which does not affect the activity) | description - | description DMSO solution | description - |
| form aqueous solution (dark coloration may develop upon storage, which does not affect the activity) | form DMSO solution | form DMSO solution | form powder |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: 0.1 g/mL, clear, colorless |
| input general use sample(s) | input general use sample(s) | input general use sample(s) | input general use sample(s) |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Klasa składowania
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Wykazy regulacyjne
Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.
288-32-4
CAS No.
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What is the concentration of the inhibitor solution? 100x?
1 answer-
Helpful?
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Does this contain any metal chelators?
1 answer-
This phosphatase inhibitor cocktail does not contain a chelator. A phosphatase inhibitor cocktail that includes EDTA is not offered at this time. Protease inhibitor cocktails with chelators are offered.
Helpful?
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