This endoproteinase is not sterile and has not been tested for suitability in cell culture. It is recommended for use in protein sequencing assays.
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P6181
Glu-C Protease
Cleaves at glutamic acid residues, suitable for Mass Spectrometry, from Staphylococcus aureus V8
Synonim(y):
V8 Protease
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
50 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1000,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
200-350-6
MDL number:
Numer WE:
eCl@ss:
32160410
1000,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
For a suitable alternative that ships today, try 324713-50UG.
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Pozwól nam pomócNazwa produktu
Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder
grade
Proteomics Grade
Quality Segment
form
lyophilized powder
analyte chemical class(es)
amino acids
technique(s)
protein mass spectrometry: suitable
suitability
suitable for protein sequencing
storage temp.
−20°C
Application
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:
- obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.
- the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
- limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
- the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.
Biochem/physiol Actions
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues. The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH 4.0) or ammonium bicarbonate (pH 7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH 7.8) Glu-C will cleave at either site. Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.
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Ta pozycja | |||
|---|---|---|---|
| description suitable for protein sequencing, lyophilized powder | description Type XVII-B, lyophilized powder, 500-1,000 units/mg solid | description - | description - |
| grade Proteomics Grade | grade - | grade Proteomics Grade | grade - |
| suitability suitable for protein sequencing | suitability - | suitability - | suitability - |
| form lyophilized powder | form lyophilized powder | form lyophilized | form lyophilized solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| analyte chemical class(es) amino acids | analyte chemical class(es) - | analyte chemical class(es) - | analyte chemical class(es) - |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Hi, is this enzyme sterile and suitable for cell culture? Thank you.
1 answer-
Helpful?
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Hi, I was just wondering you can give me the amino acid sequence for this protein. I can't find it on the page.
1 answer-
The product does not need to be sequenced to get released. Unfortunately there is no in-house information about product sequence.
Helpful?
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