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P6675
Prolidase from porcine kidney
lyophilized powder, ≥100 units/mg protein
Synonim(y):
Aminoacyl-L-proline hydrolase, Imido Dipeptidase, Prolidase, Proline dipeptidase
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
250 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1570,00 zł |
1000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4630,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-791-5
MDL number:
Numer WE:
Specific activity:
≥100 units/mg protein
1570,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócform
lyophilized powder
Quality Segment
specific activity
≥100 units/mg protein
composition
Protein, 20-74% Lowry
storage temp.
−20°C
General description
Prolidase is a cytosolic exopeptidase. It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function.
Application
Prolidase from porcine kidney has been used:
- in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides
- in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids
- to determine its effect on the activity of enterococcin A 2000
Prolidase has an important role in recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins.
Biochem/physiol Actions
Rare mutation in prolidase gene causes deficiency leading to massive imidodipeptiduria, elevated proline-containing dipeptides in plasma, recurrent infections, mental retardation and skin lesions.
Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.
Physical form
Supplied as a lyophilized powder containing Tris buffer salt and MnCl2.
Other Notes
One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C.
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Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder, ≥100 units/mg protein | description ≥0.05 unit/mg solid | description powder, ≥1.5 units/mg solid | description lyophilized powder, ≥10 units/mg protein (Bradford) |
| specific activity ≥100 units/mg protein | specific activity ≥0.05 unit/mg solid | specific activity ≥1.5 units/mg solid | specific activity ≥10 units/mg protein (Bradford) |
| form lyophilized powder | form solid | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, 20-74% Lowry | composition - | composition - | composition - |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Narządy docelowe
Respiratory system
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 1
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