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RAB0385

Atrial Natriuretic Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonim(y):

Natriuretic peptides A, Prepronatriodilatin, ANP

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Rozmiar/SKUDostępnośćCena netto
1 kit
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2030,00 zł

Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
41116158

2030,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

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species reactivity

mouse, human, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NPPA(4878)

General description

The ANP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting ANP peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANP-1 targets the common sequence of human, mouse and rat, and thus may be used to detect ANP expression in all these species with high specificity and sensitivity.

Immunogen

Atrial Natriuretic Peptide, synthetic peptide

Application

Atrial Natriuretic Peptide EIA Kit has been used for the quantification of atrial natriuretic peptide (ANP) in cell culture.
For research use only. Not for use in diagnostic procedures.
Atrial Natriuretic Peptide EIA Kit has been used to measure ANP levels in diabetes mellitus patients.

Biochem/physiol Actions

Atrial natriuretic peptide (ANP) is a polypeptide hormone released in response to atrial dilation and it stimulates diuresis, natriuresis and vasorelaxation. ANP acts as an inhibitor of renin-angiotensin-aldosterone system (RAAS) pathway. It also has a major role in body fluid homeostasis and maintenance of blood pressure. Binding of ANP to receptors on target cell stimulate the production of cyclic guanosine monophosphate (GMP). Overexpression of ANP leads to congestive heart failure in the human. Experimental studies show that ANP can be used as a therapeutic for stimulating angiogenesis in ischemic tissues. N-terminal ANP prohormone functions as a biomarker for asymptomatic systolic left ventricular (LV) dysfunction, congestive heart failure and cardiovascular mortality.
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Ta pozycja
CBL66MABC1032RAB0386
description

for serum, plasma, culture supernatant and cell lysates

description

clone A6, Chemicon®, from mouse

description

clone 11E3.9, from mouse

description

for serum, plasma, culture supernatant and cell lysates

detection method

colorimetric

detection method

-

detection method

-

detection method

colorimetric

Gene Information

human ... NPPA(4878)

Gene Information

human ... NPPA(4878)

Gene Information

human ... NPPA(4878)

Gene Information

human ... NPPB(4879)

technique(s)

enzyme immunoassay: suitable, ELISA: suitable

technique(s)

radioimmunoassay: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

western blot: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

enzyme immunoassay: suitable, ELISA: suitable

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

rat, human

species reactivity

human, mouse, rat

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

input

-

input

-

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

assay range

-

assay range

-

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL


Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki & Cennik

  • HRP-Streptavidin
    Karta charakterystyki

Piktogramy

Health hazard

Klasa składowania

10 - Combustible liquids

Słowo ostrzegawcze

Danger

Kody zagrożeń

Ostrzeżenia

Hazard Classifications

Resp. Sens. 1



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. can this product be used in porcine plasma?

    1 answer
    1. This kit has not been tested for use with Porcine plasma. The end user would have to determine suitability. Note that in the publication provided in the link below, the sequence of mature circulating Atrial Natriuretic Peptide (ANP) in human and porcine is identical.
      https://pmc.ncbi.nlm.nih.gov/articles/PMC4855512/

      Helpful?

  2. Does this ELISA measure N-terminal or C-terminal ANP?

    1 answer
    1. The ANP EIA kit can detect both N- and C- terminal since ANP has only 28 peptides.

      Helpful?

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